来自菠菜的硝酸还原酶。黄素腺嘌呤二核苷酸（FAD）与对羟基汞苯甲酸处理后酶的再激活作用。
Nitrate reductase from Spinacea oleracea. FAD and the reactivation of the enzyme treated with p-Hydroxymercuribenzoate.
作者信息
Castillo F, de la Rosa F F, Palacián E
出版信息
Rev Esp Fisiol. 1977 Dec;33(4):287-90.
PMID:594486
Abstract
Spinach nitrate reductase complex previously inactivated by treatment with mercurials p-hydroxymercuribenzoate or p-hydroxymercuriphenyl sulphonate can be reactivated by incubation with dithioerythritol. The reactivation of NADH-diaphorase seems to be FAD-dependent, whereas that of FNH2-nitrate reductase is not. The requirement of FAD for NADH-inactivation of nitrate reductase treated with p-hydroxymercuribenzoate disappears after treatment with dithioerythritol.
摘要
菠菜硝酸还原酶复合体先前经对羟基汞苯甲酸酯或对羟基汞苯磺酸盐处理而失活，可通过与二硫苏糖醇温育而重新激活。NADH-黄递酶的重新激活似乎依赖于黄素腺嘌呤二核苷酸（FAD），而FNH₂-硝酸还原酶的重新激活则不然。用二硫苏糖醇处理后，对羟基汞苯甲酸酯处理的硝酸还原酶NADH失活对FAD的需求消失。
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