发育中大鼠大脑提取物的氨基酸掺入情况。
Amino acid incorporation by preparations from the developing rat brain.
作者信息
Adams D H, Lim L
出版信息
Biochem J. 1966 May;99(2):261-5. doi: 10.1042/bj0990261.
Abstract
- A study has been made of the ability of cerebral microsome-cell sap systems, taken from rats at various ages, to incorporate [(14)C]valine. 2. The systems appear to follow Michaelis-Menten kinetics when cell sap is considered as substrate, microsomes as enzyme and total counts/mg. of microsomal protein as a measure of reaction velocity. 3. Reproducible ;affinity constant' values are obtained, and the system from 4-day-old rats has a higher V(max.) and affinity constant than the system from adult rats. 4. It is suggested the the amino acid-incorporating ability of different systems may be compared by this means.
摘要
- 对取自不同年龄大鼠的脑微粒体-细胞液系统掺入[¹⁴C]缬氨酸的能力进行了研究。2. 当将细胞液视为底物、微粒体视为酶且以每毫克微粒体蛋白的总计数作为反应速度的度量时，这些系统似乎遵循米氏动力学。3. 获得了可重复的“亲和常数”值，4日龄大鼠的系统比成年大鼠的系统具有更高的V(max.)和亲和常数。4. 有人提出，可通过这种方法比较不同系统掺入氨基酸的能力。
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