Wessman G E
Appl Microbiol. 1966 Jul;14(4):597-602. doi: 10.1128/am.14.4.597-602.1966.
A chemically defined medium was developed for the aerobic cultivation of Pasteurella haemolytica. Studies on the growth of strain H44L were conducted in a medium consisting of 15 amino acids, inorganic salts, citrate, nicotinamide, pantothenate, thiamine or thiamine monophosphate, and carbon sources. The amino acids were provided as l isomers, because racemic mixtures of some amino acids inhibited growth. The carbon source consisted of a mixture of 1.0% d-galactose and 0.1% d-glucose. Culture populations of strain H44L reached 2 x 10(10) cells per milliliter after 16 hr of incubation at 37.5 C. Other strains of P. haemolytica, from a wide variety of sources, were tested for growth in the medium, and 23 of 24 strains grew well. Five strains of P. haemolytica var. ureae failed to grow in the medium.
开发了一种用于溶血巴斯德菌需氧培养的化学成分明确的培养基。在一种由15种氨基酸、无机盐、柠檬酸盐、烟酰胺、泛酸盐、硫胺素或硫胺素单磷酸以及碳源组成的培养基中对H44L菌株的生长进行了研究。氨基酸以L型异构体形式提供，因为某些氨基酸的外消旋混合物会抑制生长。碳源由1.0%的D-半乳糖和0.1%的D-葡萄糖混合物组成。在37.5℃孵育16小时后，H44L菌株的培养物数量达到每毫升2×10(10)个细胞。对来自多种来源的其他溶血巴斯德菌菌株进行了在该培养基中生长的测试，24株菌株中有23株生长良好。5株溶血巴斯德菌脲变种在该培养基中未能生长。
