Andreeva Z M, Gridneva N I, Shobukhova T S, Akatova N S, Ershova E B
Zh Mikrobiol Epidemiol Immunobiol. 1977 Nov(11):95-7.
Differential diagnosis of enterobacteria is based on the determination of beta-galactosidase enzyme, hydrolyzing lactose in the nutrient substrates; however, the tests suggested for its determination are time consuming and their use in practice is limited. Dry nutrient medium prepared by the authors containing o-nitrophenyl-betaD-pyranoside was tested on 1625 strains of enterobacteria in comparison with the Le Minor's ONPS test used at present. The results of beta-galactosidase determination by the, mentioned methods proved to coincide (significance level greater than 95%); this permits to recommend the method described--in dry nutrient medium--as a differential-diagnostic test for the identification of enterobacteria.
肠杆菌的鉴别诊断基于对β-半乳糖苷酶的测定，该酶可水解营养底物中的乳糖；然而，用于测定该酶的现有试验耗时较长，在实际应用中受到限制。作者制备的含有邻硝基苯基-β-D-吡喃糖苷的干燥营养培养基，与目前使用的勒米诺氏邻硝基苯-β-D-吡喃半乳糖苷试验（ONPS试验）相比，对1625株肠杆菌进行了测试。通过上述两种方法测定β-半乳糖苷酶的结果被证明是一致的（显著性水平大于95%）；这使得可以推荐在干燥营养培养基中描述的方法，作为鉴定肠杆菌的鉴别诊断试验。
