Hayashi S, Nakamura S
Biochim Biophys Acta. 1976 Jun 7;438(1):37-48. doi: 10.1016/0005-2744(76)90221-7.
Glucose oxidases (beta-D-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4) from two fungal genera (Aspergillus and Penicillium) were studied chemically, physicochemically and immunologically to elucidate the similarities and dissimilarities between these enzymes. Investigation of circular dichroism spectra revealed that these enzymes proteins possess essentially identical conformations. However, differences found in thermal inactivation parameters, catalytic parameters and quantitative immunological reactivities indicate that these enzymes must have some minor but distinct variations in their structures. Interestingly, it was observed that the Penicillium enzyme cross-reacted with the antiserum against the Aspergillus enzyme with an association constant of two orders of magnitude lower than that of the Aspergillus enzyme, and that the precipitin one of the Penicillium enzyme fused together with that of the Aspergillus enzyme in the immunodouble diffusion test. These results lead to the conclusion that these enzymes are closely related but not completely identical, and suggest that they might have evolved from a common ancestral precursor.
对来自两个真菌属（曲霉属和青霉属）的葡萄糖氧化酶（β-D-葡萄糖：氧1-氧化还原酶，EC 1.1.3.4）进行了化学、物理化学和免疫学研究，以阐明这些酶之间的异同。圆二色光谱研究表明，这些酶蛋白具有基本相同的构象。然而，热失活参数、催化参数和定量免疫反应性方面的差异表明，这些酶在结构上必定存在一些微小但明显的差异。有趣的是，观察到青霉属的酶与抗曲霉属酶的抗血清发生交叉反应，其结合常数比曲霉属酶低两个数量级，并且在免疫双扩散试验中，青霉属酶的沉淀素带与曲霉属酶的沉淀素带融合在一起。这些结果得出结论，这些酶密切相关但并非完全相同，并表明它们可能是由一个共同的祖先前体进化而来。
