髓细胞中细胞质颗粒的形成。关于兔嗜异性髓细胞中细胞质颗粒形成机制的电子显微镜放射自显影研究。
Cytoplasmic granule formation in myelocytes. An electron microscope radioautographic study on the mechanism of formation of cytoplasmic granules in rabbit heterophilic myelocytes.
作者信息
Fedorko M E, Hirsch J G
出版信息
J Cell Biol. 1966 May;29(2):307-16. doi: 10.1083/jcb.29.2.307.
Abstract
The intracellular flow of tritiated lysine as revealed by electron microscope radioautography was studied in heterophilic myelocytes of rabbit marrow. Label over the Golgi complex rose to a maximum of 37% of total cytoplasmic grains 30 min after initial exposure to the tracer and fell to 11% after 3 to 4 hr of incubation. Coincident with decrease in label over the Golgi complex, grain counts over granules rose to 32% after 3 to 4 hr. The time sequence of incorporation and flow of tritiated lysine and the per cent distribution of label was similar in bone marrow myelocytes under in vivo and in vitro conditions. The results demonstrate a function of the Golgi complex in incorporating or packaging certain basic amino acids or proteins into cytoplasmic granules of heterophilic myelocytes.
摘要
利用电子显微镜放射自显影技术，对兔骨髓嗜异性髓细胞中氚标记赖氨酸的细胞内流动情况进行了研究。最初接触示踪剂30分钟后，高尔基体上的标记物升至细胞质颗粒总数的37%，达到最大值，孵育3至4小时后降至11%。与高尔基体上标记物减少的同时，颗粒上的银粒计数在3至4小时后升至32%。在体内和体外条件下，骨髓髓细胞中氚标记赖氨酸的掺入和流动的时间顺序以及标记物的百分比分布相似。结果表明，高尔基体具有将某些碱性氨基酸或蛋白质掺入或包装到嗜异性髓细胞的细胞质颗粒中的功能。
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