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大鼠肝脏线粒体的定量研究。干质量、湿质量、体积及固体浓度。

Quantitative study of mitochondria in rat liver. Dry mass, wet mass, volume, and concentration of solids.

作者信息

Glas U, Bahr G F

出版信息

J Cell Biol. 1966 Jun;29(3):507-23. doi: 10.1083/jcb.29.3.507.

DOI:10.1083/jcb.29.3.507
PMID:5962940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2106972/
Abstract

The dry mass, wet mass, and volume of mitochondria of normal rat liver were determined, as well as nitrogen content and concentration. A scheme of multiple approaches to these quantities was devised, permitting comparison of values obtained by independent methods. The following basic values are considered highly accurate: Mean dry mass, 13.6 x 10(-14) g; mean wet mass, 51.8 x 10(-14) g; mean volume, 0.43 micro(3); nitrogen content, 1.75 x 10(-14) g The work emphasizes the fact that in mitochondria the quantities, mass, and volume occur in logarithmic-normal distributions.

摘要

测定了正常大鼠肝脏线粒体的干质量、湿质量和体积,以及氮含量和浓度。设计了一种对这些量进行多种测量方法的方案,以便比较通过独立方法获得的值。以下基本值被认为是高度准确的:平均干质量,13.6×10⁻¹⁴克;平均湿质量,51.8×10⁻¹⁴克;平均体积,0.43立方微米;氮含量,1.75×10⁻¹⁴克。这项研究强调了这样一个事实,即线粒体中的质量、体积等参数呈现对数正态分布。

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本文引用的文献

1
Inability of x-rays to alter the permeability of mitochondria.X射线无法改变线粒体的通透性。
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Study of mitochondria in rat liver. Quantitative electron microscopy.大鼠肝脏线粒体的研究。定量电子显微镜检查。
J Cell Biol. 1962 Dec;15(3):489-501. doi: 10.1083/jcb.15.3.489.
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Quantitative changes in rat liver mitochondria following whole body irradiation.全身照射后大鼠肝脏线粒体的定量变化
Exp Cell Res. 1959 Jan;16(1):15-23. doi: 10.1016/0014-4827(59)90191-0.
4
The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium.通过快速离心转移至固定介质来研究线粒体内部溶质的稳态浓度。
Biochem J. 1957 May;66(1):79-91. doi: 10.1042/bj0660079.
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A method for the counting of mitochondria.一种线粒体计数方法。
Exp Cell Res. 1966 Jan;41(1):99-108. doi: 10.1016/0014-4827(66)90549-0.
6
A simple method for the determination of water content and protein concentration in subcellular elements.一种测定亚细胞成分中水分含量和蛋白质浓度的简单方法。
J Histochem Cytochem. 1966 Jan;14(1):40-4. doi: 10.1177/14.1.40.