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原生质体形成与酵母细胞壁结构。蜗牛(玛瑙螺)酶的作用。

Protoplast formation and yeast cell-wall structure. The action of the enzymes of the snail, Helix pomatia.

作者信息

Anderson F B, Millbank J W

出版信息

Biochem J. 1966 Jun;99(3):682-7. doi: 10.1042/bj0990682.

Abstract
  1. The digestive juice of the snail Helix pomatia was used in the study of the degradation of isolated cell-wall preparations from a strain of Saccharomyces carlsbergensis. 2. The crude enzyme system was fractionated by gel filtration and the activities of the more specific fractions thus obtained were examined. 3. Results are discussed with respect to (a) the nature of various factors that are essential for protoplast formation and cell-wall dissolution and (b) structures envisaged in yeast cell walls that are responsible for the observed variations in susceptibility to attack by snail juice.
摘要
  1. 本研究使用了苹果蜗牛的消化液来研究卡尔斯伯酵母菌株分离细胞壁制剂的降解情况。2. 通过凝胶过滤对粗酶系统进行分级分离,并检测由此获得的更具特异性级分的活性。3. 针对以下方面对结果进行了讨论:(a) 原生质体形成和细胞壁溶解所必需的各种因素的性质;(b) 酵母细胞壁中设想的结构,这些结构导致了观察到的对蜗牛消化液攻击敏感性的差异。

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