Viviani R, Sechi A M, Lenaz G
J Lipid Res. 1966 Jul;7(4):473-8.
Rats were fed a low protein diet deficient in and supplemented with lysine and threonine. Liver lipids contained more lecithin, sphingomyelin, and free fatty acids, and less amino phospholipids in the deficient rats. No variations in fatty acid composition of choline- and ethanolamine-containing phospholipids were found; only palmitic acid was increased in the serine-containing phospholipids of the deficient animals. The incorporation of acetate-(14)C into phospholipids, but not into other liver lipids, was lower in deficient rats. In the plasma of deficient rats the concentration of esterified fatty acids and phospholipids was lower, of free fatty acids higher, than in the controls. The fatty acid composition of depot fat differed from that of liver neutral fat both in deficient and supplemented animals. The results presented establish that multiple metabolic defects resulting from lysine and threonine deficiency accompany the fatty liver. The design of the experiments does not permit conclusions to be drawn regarding the causal relationship between the various alterations in lipid metabolism and the fatty liver.
给大鼠喂食缺乏赖氨酸和苏氨酸并补充了这两种氨基酸的低蛋白饮食。缺乏这些氨基酸的大鼠肝脏脂质中卵磷脂、鞘磷脂和游离脂肪酸含量更多，氨基磷脂含量更少。未发现含胆碱和乙醇胺的磷脂的脂肪酸组成有变化；仅缺乏氨基酸的动物含丝氨酸的磷脂中棕榈酸增加。缺乏氨基酸的大鼠肝脏中，乙酸 -（14）C掺入磷脂而非其他肝脏脂质的量较低。与对照组相比，缺乏氨基酸的大鼠血浆中酯化脂肪酸和磷脂浓度较低，游离脂肪酸浓度较高。在缺乏和补充氨基酸的动物中，储存脂肪的脂肪酸组成与肝脏中性脂肪不同。所呈现的结果表明，赖氨酸和苏氨酸缺乏导致的多种代谢缺陷与脂肪肝相伴。实验设计不允许就脂质代谢的各种改变与脂肪肝之间的因果关系得出结论。
