Menkes J H, Philippart M, Concone M C
J Lipid Res. 1966 Jul;7(4):479-86.
The fatty acid composition of cerebrosides and sulfatides from frontal lobe gray and white matter was determined for five fresh and four formalinized adult brains and for eight infants. Fatty acid patterns were unaffected by formalinization, but varied considerably from one another in the proportion of saturated to unsaturated fatty acids. The percentages of 24:0 and 24:1 increased with age. Cerebrosides obtained from areas such as the brainstem and cerebellum, where myelination was more advanced, tended to have a larger proportion of long-chain fatty acids than samples extracted from frontal or parietal lobe white matter. Hydroxy fatty acids showed an adult pattern in all instances in which amounts sufficient for accurate quantification could be isolated. Lipid hexose, cerebroside + sulfatide hexose, and methanoleluted hexose were measured in the brains of 12 infants ranging in age from a 4 month fetus to 2 yr. In the most immature, the majority of lipid hexose was in the form of glycolipids more polar than cerebrosides and sulfatides. These have tentatively been identified as hematosides and globosides. With maturation, cerebrosides and sulfatides increased progressively, but the amounts of the more polar glycolipids remained constant in relation to the total lipid content of tissue.
测定了5个新鲜成人脑和4个经福尔马林固定的成人脑以及8个婴儿脑额叶灰质和白质中脑苷脂和硫脂的脂肪酸组成。脂肪酸模式不受福尔马林固定的影响，但饱和脂肪酸与不饱和脂肪酸的比例彼此差异很大。24:0和24:1的百分比随年龄增长而增加。从脑干和小脑等髓鞘形成更发达的区域获得的脑苷脂，其长链脂肪酸的比例往往比从额叶或顶叶白质中提取的样本更大。在所有能够分离出足够准确定量的羟基脂肪酸的情况下，其呈现出成人模式。在年龄从4个月胎儿到2岁的12名婴儿的脑中测量了脂质己糖、脑苷脂+硫脂己糖和甲醇洗脱己糖。在最不成熟的脑中，大多数脂质己糖是以比脑苷脂和硫脂极性更强的糖脂形式存在。这些糖脂初步被鉴定为血苷脂和球苷脂。随着成熟，脑苷脂和硫脂逐渐增加，但极性更强的糖脂的量相对于组织的总脂质含量保持恒定。
