来自葡萄糖的乙酰基在离体灌注山羊乳腺中的命运。
The fate of acetyl groups derived from glucose in the isolated perfused goat udder.
作者信息
Hardwick D C
出版信息
Biochem J. 1966 Apr;99(1):228-31. doi: 10.1042/bj0990228.
Abstract
- In the isolated perfused goat mammary gland, both glucose and acetate contribute 20-30% of carbon to citrate via acetyl-CoA. 2. Carbon dioxide does not contribute significantly to the formation of acetyl-CoA. 3. ATP citrate lyase, which, in rats, yields acetyl-CoA for fatty acid synthesis, is absent from or very low in high-speed supernatant fraction of ruminant tissue. 4. It is reasonable to infer that intramitochondrial acetyl-CoA, to which both glucose and acetate contribute, is separated from extramitochondrial acetyl-CoA to which glucose does not contribute and which is used for fatty acid synthesis.
摘要
- 在离体灌注的山羊乳腺中，葡萄糖和乙酸盐通过乙酰辅酶A为柠檬酸提供20%-30%的碳。2. 二氧化碳对乙酰辅酶A的形成贡献不大。3. 在大鼠中产生用于脂肪酸合成的乙酰辅酶A的ATP柠檬酸裂解酶，在反刍动物组织的高速上清液部分中不存在或含量极低。4. 合理推断，葡萄糖和乙酸盐都能提供的线粒体内乙酰辅酶A，与葡萄糖不能提供的、用于脂肪酸合成的线粒体外乙酰辅酶A是分开的。
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本文引用的文献
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The metabolism of acetate and glucose by the isolated perfused udder. 2. The contribution of acetate and glucose to carbon dioxide and milk constituents.离体灌注乳腺中乙酸盐和葡萄糖的代谢。2. 乙酸盐和葡萄糖对二氧化碳及乳汁成分的贡献。
Biochem J. 1963 Aug;88(2):213-20. doi: 10.1042/bj0880213.
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Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates.克氏梭菌酶制剂催化的脂肪酸合成。VI. 酰基磷酸酯的反应。
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Chemical degradation of 14C-labeled glutamic acid.14C标记的谷氨酸的化学降解
Biochim Biophys Acta. 1961 Sep 16;52:390-2. doi: 10.1016/0006-3002(61)90693-x.
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Methods for determination of coenzyme A.辅酶A的测定方法。
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Biochem J. 1965 Jan;94(1):209-15. doi: 10.1042/bj0940209.
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