Nwangwu P U, Holcslaw T L, Stohs S J
Arch Int Pharmacodyn Ther. 1977 Oct;229(2):219-26.
The search for new and better antiarrhythmic agents is complicated by the fact that currently there is no generally accepted, rapid, highly reproducible, in vivo screening technique which allows the use of large numbers of small animals for the purpose of statistical evaluation. Most of the methods available for use with small animals employ visual observation of the animal to assess electrophysiological events occurring in the heart. A rapid, inexpensive, in vivo screening technique is discribed, using aconitine as the arrhythmogenic agent. Arrhythmias induced by aconitine were measured by electrocardiogram (lead II). The times to onset of initial cardiac arrhythmias and ventricular tachycardia were determined and compared for significance in animals pretreated with physiological saline (control group), standard antiarrhythmic agents, and two drugs lacking antiarrhythmic activity. The results indicate that the method is a sensitive, rapid technique for preliminary in vivo screening of antiarrhythmic drugs.
寻找新型且更好的抗心律失常药物面临着复杂的情况，原因在于目前没有一种被广泛接受的、快速的、高度可重复的体内筛选技术，能够使用大量小动物进行统计评估。大多数适用于小动物的方法是通过对动物进行视觉观察，以评估心脏中发生的电生理事件。本文描述了一种快速、廉价的体内筛选技术，使用乌头碱作为致心律失常剂。通过心电图（II导联）测量乌头碱诱导的心律失常。测定并比较生理盐水预处理动物（对照组）、标准抗心律失常药物以及两种无抗心律失常活性药物的动物出现初始心律失常和室性心动过速的时间，以确定其显著性。结果表明，该方法是一种用于抗心律失常药物初步体内筛选的灵敏、快速技术。
