Holladay D W, Caton J E, Ball F L, Holleman J W, Anderson N G
Immunol Commun. 1976;5(1-2):1-11. doi: 10.3109/08820137609020608.
Antisera against human placental proteins were developed in goats and rabbits, using immunoadjuvants and a prolonged injection schedule. The antisera were absorbed with normal serum proteins and then tested in immunodiffusion against normal and pregnancy sera. Two bands of precipitation due to pregnancy antigens were observed in pregnancy sera as early as 18 days after conception. Detection of these antigens has possibilities for application as an early pregnancy test.
利用免疫佐剂和延长注射方案，在山羊和兔子体内制备了抗人胎盘蛋白抗血清。这些抗血清用正常血清蛋白进行吸收，然后在免疫扩散试验中与正常血清和妊娠血清进行检测。早在受孕后18天，在妊娠血清中就观察到了由妊娠抗原引起的两条沉淀带。检测这些抗原具有作为早孕检测方法应用的可能性。
