Smith J L, Alford J A
Appl Microbiol. 1966 Sep;14(5):699-705. doi: 10.1128/am.14.5.699-705.1966.
Addition of lard or sodium oleate to the medium used for lipase production by Pseudomonas fragi resulted in a decreased accumulation of lipase in the culture supernatant fluid without affecting cell growth. The production and activity of lipase was inhibited by lard, sodium oleate, and the salts of other unsaturated fatty acids. Some divalent cations, Tweens, lecithin, and bovine serum prevented oleate inhibition, but did not reverse it. Similar inhibitory actions were observed with Geotrichum candidum lipase, but not with a staphylococcal lipase or pancreatic lipase. A protective effect by protein in crude enzyme preparations is indicated. The ability of oleate to lower surface tension does not appear to be related to its ability to inhibit lipase.
在用于脆弱假单胞菌生产脂肪酶的培养基中添加猪油或油酸钠，会导致脂肪酶在培养上清液中的积累减少，而不影响细胞生长。猪油、油酸钠和其他不饱和脂肪酸的盐会抑制脂肪酶的产生和活性。一些二价阳离子、吐温、卵磷脂和牛血清可防止油酸盐的抑制作用，但不能逆转这种抑制。对于白色念珠菌脂肪酶也观察到类似的抑制作用，但葡萄球菌脂肪酶或胰脂肪酶则没有。粗酶制剂中的蛋白质显示出保护作用。油酸盐降低表面张力的能力似乎与其抑制脂肪酶的能力无关。
