Foulkes E C
J Gen Physiol. 1966 Sep;50(1):1-8. doi: 10.1085/jgp.50.1.1.
The isotopic equilibration of urea, thiourea, and inulin between urine and plasma was determined in rabbits in the presence or absence of antidiuretic hormone (ADH). Animals were anesthetized with ethanol and permitted to reach steady state after completion of surgery. Tracer was then administered by intraarterial infusion in such a manner that a high constant specific activity in plasma was rapidly attained. Urine flow was kept independent of ADH by addition of mannitol. Urea/creatinine clearance ratios and the accumulation of urea in renal medulla and papilla also remained unaffected by ADH. Under these conditions, thiourea and inulin at all times approached equilibrium, at similar rates. In the absence of ADH, urea also equilibrated at a rate similar to that of inulin. The addition of ADH, however, significantly prolonged the delay before urinary urea reached the high constant specific activity of plasma urea. These observations are interpreted in terms of a specific effect of the hormone on the solute permeability of the nephron.
在有或没有抗利尿激素（ADH）的情况下，测定了兔尿液与血浆中尿素、硫脲和菊粉的同位素平衡。动物用乙醇麻醉，术后待其达到稳态。然后通过动脉内输注给予示踪剂，以便迅速使血浆达到高恒定比活度。通过添加甘露醇使尿流与ADH无关。尿素/肌酐清除率以及尿素在肾髓质和乳头中的蓄积也不受ADH影响。在这些条件下，硫脲和菊粉始终以相似的速率接近平衡。在没有ADH的情况下，尿素也以与菊粉相似的速率达到平衡。然而，添加ADH显著延长了尿尿素达到血浆尿素高恒定比活度之前的延迟时间。这些观察结果被解释为该激素对肾单位溶质通透性的特定作用。
