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一种用于检测血浆中可溶性纤维蛋白的灵敏乳胶凝集法。

A sensitive latex agglutination method for the detection of soluble fibrin in plasma.

作者信息

Müller U P, Largo R, Straub P W

出版信息

Blut. 1977 Dec;35(6):465-79. doi: 10.1007/BF00996693.

DOI:10.1007/BF00996693
PMID:597619
Abstract

A sensitive method is described for the rapid detection of soluble fibrin in human plasma. It is based on the agglutination of Latex particles suspended in a solution of purified fibrin monomers and Blue Dextran 2000. Photometric registration of the decrease in optical density (OD) during agglutination allows the results to be quantitated. The deltaOD depends on the ratio of fibrin monomers to fibrinogen in the tested plasma rather than on the absolute amount of fibrin monomers present.

摘要

本文描述了一种用于快速检测人血浆中可溶性纤维蛋白的灵敏方法。该方法基于悬浮在纯化纤维蛋白单体溶液和蓝色葡聚糖2000中的乳胶颗粒的凝集反应。通过光度法记录凝集过程中光密度(OD)的降低,可对结果进行定量分析。光密度变化值(deltaOD)取决于被测血浆中纤维蛋白单体与纤维蛋白原的比例,而非纤维蛋白单体的绝对含量。

相似文献

1
A sensitive latex agglutination method for the detection of soluble fibrin in plasma.一种用于检测血浆中可溶性纤维蛋白的灵敏乳胶凝集法。
Blut. 1977 Dec;35(6):465-79. doi: 10.1007/BF00996693.
2
[A sensitive latex agglutination method for the determination of soluble fibrin in plasma].
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3
Detection of soluble intermediates of the fibrinogen-fibrin conversion using erythrocytes coated with fibrin monomers.使用包被有纤维蛋白单体的红细胞检测纤维蛋白原 - 纤维蛋白转化的可溶性中间体。
Blood. 1976 Jun;47(6):991-1002.
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Rapid detection of cross-linked fibrin degradation products in plasma using monoclonal antibody-coated latex particles.使用单克隆抗体包被的乳胶颗粒快速检测血浆中的交联纤维蛋白降解产物。
Am J Clin Pathol. 1986 Mar;85(3):360-4. doi: 10.1093/ajcp/85.3.360.
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Latex immunoturbidimetric assay for soluble fibrin complex.可溶性纤维蛋白复合物的乳胶免疫比浊法
Clin Chem. 2005 Jan;51(1):183-8. doi: 10.1373/clinchem.2004.040360. Epub 2004 Oct 28.
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Fibrin degradation products in the postoperative period. Evaluation of a new latex agglutination method.
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The detection of fibrinogen-fibrin degradation products by means of a new antibody-coated latex particle.采用新型抗体包被乳胶颗粒检测纤维蛋白原 - 纤维蛋白降解产物。
J Clin Pathol. 1972 Aug;25(8):680-2. doi: 10.1136/jcp.25.8.680.
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Measurement of soluble fibrin monomer-fibrinogen complex in plasmas derived from patients with various underlying clinical situations.对患有各种潜在临床病症患者的血浆中可溶性纤维蛋白单体-纤维蛋白原复合物的测定。
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Detection of serum fibrinogen and fibrin degradation products. Comparison of six technics using purified products and application in clinical studies.血清纤维蛋白原和纤维蛋白降解产物的检测。六种使用纯化产物的技术比较及其在临床研究中的应用。
Am J Med. 1971 Jul;51(1):71-82. doi: 10.1016/0002-9343(71)90325-1.

本文引用的文献

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Influence of dextran on the conversion of fibrinogen to fibrin.右旋糖酐对纤维蛋白原向纤维蛋白转化的影响。
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6
Acceleration of fibrin polymerization by dextran and ficoll. Interaction with calcium and plasma proteins.右旋糖酐和聚蔗糖对纤维蛋白聚合的加速作用。与钙及血浆蛋白的相互作用。
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Gelation of soluble fibrin in plasma by ethanol.乙醇使血浆中的可溶性纤维蛋白发生凝胶化。
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Familial disturbance of fibrin monomer aggregation.
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N-terminal analysis during coagulation of purified human fibrinogen, fraction I, and plasma.纯化人纤维蛋白原I组分及血浆凝血过程中的N端分析。
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