Turner R T, Schraer H
Calcif Tissue Res. 1977 Dec 28;24(2):157-62. doi: 10.1007/BF02223310.
Medullary bone was induced in male Japanese quail by administration of estradiol valerate. An increase in the organic weight of the femur was observed by 36 h after estrogen and an increase in ash weight was observed by 96 h. A complex sequence of metabolic changes in the femur occurred after estrogen treatment. A large increase in uptake of 3H-proline associated with enhanced collagen formation began 36 h after estrogen and reached a peak 3.5 times the control rate at 60 h. The onset of mineralization of the newly formed bone matrix, as determined by 45Ca uptake, began about 24 h after onset of collagen synthesis indicating that synthesis of the bone matrix and its mineralization occur sequentially. Large increases in the rates of uptake of 3H-uridine and 3H-thymidine occurred prior to the onset of medullary bone formation and appear to reflect the differentiation of osteogenic precursors to bone-forming cells.
通过给予戊酸雌二醇，在雄性日本鹌鹑中诱导出了骨髓骨。雌激素处理36小时后观察到股骨有机重量增加，96小时后观察到灰分重量增加。雌激素处理后，股骨发生了一系列复杂的代谢变化。与胶原蛋白形成增强相关的3H-脯氨酸摄取在雌激素处理后36小时开始大幅增加，并在60小时达到峰值，是对照速率的3.5倍。通过45Ca摄取确定，新形成的骨基质矿化开始于胶原蛋白合成开始后约24小时，这表明骨基质的合成及其矿化是依次发生的。在骨髓骨形成开始之前，3H-尿苷和3H-胸苷的摄取速率大幅增加，这似乎反映了成骨前体细胞向成骨细胞的分化。
