Seyer J M, Glimcher M J
Calcif Tissue Res. 1977 Dec 29;24(3):253-7. doi: 10.1007/BF02223325.
The proteins and peptides of immature enamel were extracted from freshly slaughtered bovine embryos in solutions containing protease inhibitors. No detectable differences were noted in the number of components, their overall amino acid composition, or molecular weights from the proteins and peptides extracted 12-16 h postmortem in solutions which contained no protease inhibitors. These data indicate that the large number of components found in developing bovine enamel is not due to proteolysis occurring during their isolation. Significant amounts of protein components having molecular weights greater than approximately 15,000 were not detected. Therefore, if the ameloblasts initially synthesize only a few high molecular weight protein species, the present data imply that in vivo degradation of the high molecular weight enamel proteins occurs very rapidly after their synthesis and precedes the massive loss of protein which accompanies the final stages of enamel mineralization and maturation.
未成熟牙釉质的蛋白质和肽是从刚宰杀的牛胚胎中，在含有蛋白酶抑制剂的溶液中提取的。与在不含蛋白酶抑制剂的溶液中死后12 - 16小时提取的蛋白质和肽相比，在成分数量、整体氨基酸组成或分子量方面未发现可检测到的差异。这些数据表明，在发育中的牛牙釉质中发现的大量成分并非由于其分离过程中发生的蛋白水解作用。未检测到大量分子量大于约15,000的蛋白质成分。因此，如果成釉细胞最初仅合成少数几种高分子量蛋白质种类，那么目前的数据表明，高分子量牙釉质蛋白在体内合成后很快就会发生降解，并且在牙釉质矿化和成熟的最后阶段伴随的大量蛋白质损失之前就已发生。
