Aickin C M, Fraser S, Cooper E, Hall G, Burke C W
Clin Endocrinol (Oxf). 1977 Dec;7(6):469-79. doi: 10.1111/j.1365-2265.1977.tb01339.x.
An accurate and reproducible method for measurement of radioactive species in blood after in vivo injection of labelled iodothyronines is described. By extraction with high-affinity antisera, radioactive reverse T3 and T3 are separated from serum quantitatively. Radioiodide is quantitatively separated from radio-thyronine species and serum proteins by Sephadex G50 filtration. The residual mixture of radio-T4 and iodoprotein is quantitatively resolved by ion-exchange adsorption. Minimal misclassification of radiospecies occurs, and can be corrected for. Mean recoveries of various radiospecies added to serum were: radioiodide 98.9%, radio-rT3 87.6%, radio-L-T3 94.5%, radio-T4 98.0% and radioiodoprotein 94.5%. The performance of the method is superior to that of chemical methods such as trichloracetic acid precipitation, ion-exchange or alkaline Sephadex extraction, and chromatographic separation.
本文描述了一种在体内注射标记碘甲状腺原氨酸后测量血液中放射性物质的准确且可重复的方法。通过用高亲和力抗血清提取，放射性反三碘甲状腺原氨酸（reverse T3）和三碘甲状腺原氨酸（T3）可从血清中定量分离。通过葡聚糖凝胶G50过滤，放射性碘化物可从放射性甲状腺原氨酸和血清蛋白中定量分离。放射性甲状腺素（radio-T4）和碘蛋白的残留混合物通过离子交换吸附进行定量分离。放射性物质的错误分类最少，并且可以进行校正。添加到血清中的各种放射性物质的平均回收率分别为：放射性碘化物98.9%、放射性反三碘甲状腺原氨酸87.6%、放射性左旋三碘甲状腺原氨酸94.5%、放射性甲状腺素98.0%和放射性碘蛋白94.5%。该方法的性能优于三氯乙酸沉淀、离子交换或碱性葡聚糖凝胶提取以及色谱分离等化学方法。
