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普通欧洲蝾螈南方亚种(两栖纲,有尾目)核糖体RNA基因的染色体定位。II. 18S + 28S核糖体RNA染色体位点数量和位置的种内变异性

Chromosome location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela). II. Intraspecific variability in number and position of the chromosome loci for 18S + 28S ribosomal RNA.

作者信息

Nardi I, Barsacchi-Pilone G, Batistoni R, Andronico F

出版信息

Chromosoma. 1977 Nov 3;64(1):67-84. doi: 10.1007/BF00292889.

Abstract

Ribosomal genes have been localized on mitotic and lampbrush chromosomes of 20 specimens of Triturus vulgaris meridionalis by in situ hybridization with 3H 18S + 28S rRNA. The results may be summarized as follows: 1) each individual shows positive in situ hybridization at the nucleolus organizing region (NOR) on chromosome XI; 2) in addition, many specimens exhibit a positive reaction in chromosomal sites other than the NOR (additional ribosomal sites); 3) the chromosomal distribution of the additional sites appears to be identical in different tissues from the same specimen and to follow a specific individual pattern; 4) the additional ribosomal sites are preferentially found at the telomeric, centromeric or C-band regions of the chromosomes involved.

摘要

通过用³H 18S + 28S rRNA进行原位杂交,已将核糖体基因定位在20个南欧普通蝾螈标本的有丝分裂染色体和灯刷染色体上。结果可总结如下:1)每个个体在第十一条染色体的核仁组织区(NOR)显示阳性原位杂交;2)此外,许多标本在NOR以外的染色体位点呈现阳性反应(额外核糖体位点);3)同一标本不同组织中额外位点的染色体分布似乎相同,并遵循特定的个体模式;4)额外核糖体位点优先出现在相关染色体的端粒、着丝粒或C带区域。

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