Wobus U, Serfling E
Chromosoma. 1977 Nov 30;64(3):279-86. doi: 10.1007/BF00328082.
The RNA of Balbiani ring BR2 of polytene chromosomes from Chironomus thummi salivary glands was microisolated and reassociated in the presence of an excess of total larval DNA. BR2 RNA reacts as a single component with a C0t 1/2 of 8.6. Ribosomal precursor RNA from microisolated nucleoli reassociates under identical conditions with a C0t 1/2 of 12.3. These C0t 1/2-values suggest repetition frequencies in the range of 35 and 50 for ribosomal DNA and Balbiani ring 2 DNA, respectively. The data presented here favour the view that the gene for BR2 RNA of C. thummi is internally repeated and contains only one type of DNA sequence.
从嗜尸摇蚊唾液腺的多线染色体中微分离出巴尔比亚尼环BR2的RNA，并在过量的幼虫总DNA存在下使其重新缔合。BR2 RNA作为单一成分反应，C0t 1/2为8.6。在相同条件下，微分离核仁中的核糖体前体RNA重新缔合，C0t 1/2为12.3。这些C0t 1/2值表明核糖体DNA和巴尔比亚尼环2 DNA的重复频率分别在35和50范围内。此处给出的数据支持这样一种观点，即嗜尸摇蚊BR2 RNA的基因在内部重复，且仅包含一种类型的DNA序列。
