Yokoro K, Kajihara H, Kodama Y, Nagao K, Hamada K, Kinomura A
Gan. 1977 Dec;68(6):825-8.
Chronic toxicity of 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2), which had been widely used in Japan as a food additive, was studied in both sexes of ICR/JCL mice by feeding a diet containing AF-2 in a concentration of 0.4% or 0.08%. The experiments confirmed its definite carcinogenicity in the forestomach, with dose dependency; more than 70% of mice fed the higher dose developed tumors, the majority of which were squamous cell carcinomas with metastatic growths, whereas, with a lower dose, fewer tumors occurred, following a longer latent period. In addition, possible leukemogenicity was suggested by a higher incidence in AF-2-fed females (10.8%) over that in control females (3.2%). The development of non-neoplastic lesions in the liver and kidneys, and of amyloidosis in various organs was also observed in some of AF-2-fed mice.
2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺（AF-2）曾在日本作为食品添加剂被广泛使用，本研究通过给ICR/JCL小鼠雌雄两性喂食含0.4%或0.08%浓度AF-2的饲料，对其慢性毒性进行了研究。实验证实了其在前胃具有明确的致癌性，且存在剂量依赖性；喂食高剂量AF-2的小鼠中，超过70%发生肿瘤，其中大多数为伴有转移生长的鳞状细胞癌，而喂食低剂量AF-2的小鼠发生肿瘤的数量较少，潜伏期更长。此外，喂食AF-2的雌性小鼠白血病发生率（10.8%）高于对照雌性小鼠（3.2%），提示可能存在致白血病性。在一些喂食AF-2的小鼠中，还观察到肝脏和肾脏出现非肿瘤性病变以及各器官发生淀粉样变性。
