Effect of tris (hydroxymethyl) aminomethane salt of prostaglandin F2alpha on post-thaw motility of bovine spermatozoa.

The effect of the tris (hydroxymethyl) aminomethane salt of prostaglandin F2alpha on the post-thaw motility of bovine spermatozoa was evaluated for two ejaculates from each of five bulls. This salt of prostaglandin F2alpha was added to the glycerol fraction of the extender to provide 37.5, 112.5, or 337.5 microgram of active prostaglandin F2alpha per .5-ml straw. Post-thaw progressive motility was depressed by the addition of the salt of prostaglandin F2alpha. Motilities averaged over both 0 and 2 h of post-thaw incubation were 19.3, 17.8, 13.6, and 5.8% for 0, 37.5, 112.5, and 337.5 microgram.