通过使用固定在琼脂糖凝胶上的细胞色素c来研究细胞色素c的蛋白质构象。
An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel.
作者信息
Greenwood C, Moore T A
出版信息
Biochem J. 1976 Feb 1;153(2):159-63. doi: 10.1042/bj1530159.
Abstract
Cytochrome c insolubilized on to agarose gel was shown to be more resistant to denaturation and carboxymethylation than the soluble protein. These differences are discussed both with respect to the conformational changes that take place during denaturation of cytochrome c and with respect to the pH-dependent forms of carboxymethyl-cytochrome c.
摘要
固定在琼脂糖凝胶上的细胞色素c比可溶性蛋白质对变性和羧甲基化更具抗性。这些差异既从细胞色素c变性过程中发生的构象变化方面进行了讨论，也从羧甲基化细胞色素c的pH依赖形式方面进行了讨论。
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