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通过使用固定在琼脂糖凝胶上的细胞色素c来研究细胞色素c的蛋白质构象。

An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel.

作者信息

Greenwood C, Moore T A

出版信息

Biochem J. 1976 Feb 1;153(2):159-63. doi: 10.1042/bj1530159.

DOI:10.1042/bj1530159
PMID:6001
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172558/
Abstract

Cytochrome c insolubilized on to agarose gel was shown to be more resistant to denaturation and carboxymethylation than the soluble protein. These differences are discussed both with respect to the conformational changes that take place during denaturation of cytochrome c and with respect to the pH-dependent forms of carboxymethyl-cytochrome c.

摘要

固定在琼脂糖凝胶上的细胞色素c比可溶性蛋白质对变性和羧甲基化更具抗性。这些差异既从细胞色素c变性过程中发生的构象变化方面进行了讨论，也从羧甲基化细胞色素c的pH依赖形式方面进行了讨论。

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Biochem J. 1976 Feb 1;153(2):159-63. doi: 10.1042/bj1530159.
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本文引用的文献

1
THE 695-MMM. BAND OF FERRICYTOCHROME C AND ITS RELATIONSHIP TO PROTEIN CONFORMATION.细胞色素C的695毫微米谱带及其与蛋白质构象的关系。
Biochemistry. 1964 Aug;3:1045-9. doi: 10.1021/bi00896a006.
2
Spectrum of horse-heart cytochrome c.马心细胞色素c的光谱
Biochem J. 1959 Mar;71(3):570-2. doi: 10.1042/bj0710570.
3
Production of a "cytochrome c" with myoglobin-like properties by alkylating the cyanide complex with bromoacetate.通过用溴乙酸酯烷基化氰化物配合物来制备具有肌红蛋白样性质的“细胞色素c”。
Nature. 1965 Jun 12;206(989):1150-1. doi: 10.1038/2061150a0.
4
The reversible unfolding of horse heart ferricytochrome c.马心脏铁细胞色素c的可逆去折叠
Biochemistry. 1968 Aug;7(8):2893-8. doi: 10.1021/bi00848a028.
5
Conformation of cytochromes. 3. Effect of urea, temperature, extrinsic ligands, and pH variation on the conformation of horse heart ferricytochrome c.细胞色素的构象。3. 尿素、温度、外在配体及pH变化对马心高铁细胞色素c构象的影响
Biochemistry. 1968 Feb;7(2):765-76. doi: 10.1021/bi00842a035.
6
The effects of alkylation of methionyl residues on the properties of horse cytochrome c.甲硫氨酰残基烷基化对马细胞色素c性质的影响。
J Biol Chem. 1970 Apr 10;245(7):1552-7.
7
The chemical attachment of chymotrypsin to water-insoluble polymers using 2-amino-4,6-dichloro-s-triazine.
Biochim Biophys Acta. 1970 Feb 11;198(2):276-85. doi: 10.1016/0005-2744(70)90060-4.
8
Studies on ferricytochrome c. I. Effect of pH, ionic strength and protein denaturants on the spectra of ferricytochrome c.高铁细胞色素c的研究。I. pH值、离子强度和蛋白质变性剂对高铁细胞色素c光谱的影响。
Eur J Biochem. 1971 Sep 13;22(1):5-10. doi: 10.1111/j.1432-1033.1971.tb01507.x.
9
The appearance of transient species of cytochrome c upon rapid oxidation or reduction at alkaline pH.在碱性pH值下快速氧化或还原时细胞色素c瞬态物种的出现。
J Biol Chem. 1973 Dec 10;248(23):8130-6.
10
Conformational changes upon reduction of cytochrome c.细胞色素c还原时的构象变化。
Cold Spring Harb Symp Quant Biol. 1972;36:397-404. doi: 10.1101/sqb.1972.036.01.051.