胰岛素对乳腺组织体外核糖核酸和蛋白质合成的刺激作用。

Stimulation by insulin of the formation of ribonucleic acid and protein by mammary tissues in vitro.

作者信息

Mayne R, Barry J M, Rivera E M

出版信息

Biochem J. 1966 Jun;99(3):688-93. doi: 10.1042/bj0990688.

DOI:10.1042/bj0990688

PMID:6007358

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1265059/

Abstract

Insulin is one of the hormones that are essential for successful tissue culture of explants of the mammary glands of pregnant mice. We report here effects of insulin on RNA and protein formation by mammary tissue from pregnant mice and rats incubated in tissue-culture medium 199. 2. The incorporation of [(14)C]adenine over 3hr. into the RNA of explants of the mammary glands of pregnant mice was increased by an average of 68% when the medium contained 5mug. of insulin/ml. Under similar conditions the incorporation into the RNA of slices of the glands of pregnant rats was increased by an average of 61%. Incorporation into the RNA of slices from lactating rats was stimulated to a smaller extent. 3. Adipose tissue was separated from the glands of pregnant mice and the effect of insulin on the incorporation of adenine into its RNA was studied. In whole explants the incorporation of adenine, both with and without insulin, is almost entirely into the RNA of the mammary parenchyma and not of the adipose tissue. 4. Insulin also stimulated by 38% the incorporation of [(14)C]leucine over 3hr. into the proteins of slices of the glands of pregnant rats. It had no significant effect on slices from lactating rats. 5. Actinomycin D (10mug./ml.) decreased the incorporation of [(14)C]adenine into the RNA of slices of the glands of pregnant rats by an average of 97%. Though it also decreased the incorporation of [(14)C]leucine into the proteins by an average of 25%, the percentage stimulation by insulin of this incorporation remained unchanged.

摘要

胰岛素是成功进行妊娠小鼠乳腺外植体组织培养所必需的激素之一。我们在此报告胰岛素对在199组织培养基中培养的妊娠小鼠和大鼠乳腺组织RNA和蛋白质形成的影响。2. 在含有5微克/毫升胰岛素的培养基中，妊娠小鼠乳腺外植体的RNA在3小时内对[（14）C]腺嘌呤的掺入平均增加了68%。在类似条件下，妊娠大鼠腺体切片RNA的掺入平均增加了61%。对泌乳大鼠切片RNA的掺入刺激程度较小。3. 将脂肪组织从妊娠小鼠的腺体中分离出来，研究胰岛素对腺嘌呤掺入其RNA的影响。在整个外植体中，无论有无胰岛素，腺嘌呤几乎完全掺入乳腺实质的RNA中，而非脂肪组织的RNA。4. 胰岛素在3小时内也使妊娠大鼠腺体切片蛋白质对[（14）C]亮氨酸的掺入增加了38%。对泌乳大鼠切片没有显著影响。5. 放线菌素D（10微克/毫升）使妊娠大鼠腺体切片RNA中[（14）C]腺嘌呤的掺入平均降低了97%。尽管它也使[（14）C]亮氨酸掺入蛋白质的量平均降低了25%，但胰岛素对这种掺入的刺激百分比保持不变。