从大鼠肝脏线粒体中分离并部分鉴定含多聚腺苷酸的7.5S信使核糖核酸
Isolation and partial characterization of poly (A)-containing 7.5S messenger RNA from rat liver mitochondria.
作者信息
Kisselev O I, Gaitskhoki V S, Neifakh S A
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4411-24. doi: 10.1093/nar/4.12.4411.
Poly(A)-containing low molecular weight (7.5S) messenger RNA was isolated in a highly purified form from both polyribosomes and post-polysomal supernatant of rat liver mitochondria. Both mRNA's contain rather short poly(A) tracts (40-70 mononucleotides) according to a profile of their elution from poly(U)-Sepharose column with a gradient of formamide concentration. Both mRNA's when added to a preincubated mitochondrial lysate programmed the synthesis of a hydrophobic polypeptide of a molecular weight about 9000 daltons which was soluble in the neutral chloroform-methanol mixture.
从大鼠肝线粒体的多核糖体和多核糖体后上清液中，以高度纯化的形式分离出了含聚腺苷酸的低分子量（7.5S）信使核糖核酸。根据其在甲酰胺浓度梯度下从聚尿苷酸-琼脂糖柱上洗脱的图谱，这两种信使核糖核酸都含有相当短的聚腺苷酸链（40 - 70个单核苷酸）。当将这两种信使核糖核酸添加到预先孵育的线粒体裂解物中时，它们都能指导合成一种分子量约为9000道尔顿的疏水性多肽，该多肽可溶于中性氯仿 - 甲醇混合物中。
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