Zamboni L, Mishell D R, Bell J H, Baca M
J Cell Biol. 1966 Sep;30(3):579-600. doi: 10.1083/jcb.30.3.579.
A penetrated ovum was recovered from the oviduct of a 33 year old surgical patient who had had sexual intercourse 26 hr before the operation. The ovum was in the pronuclear stage. The ooplasmic organelles were mainly represented by mitochondria, endoplasmic reticulum components, and Golgi elements. Small vesicles were found in the space between the two sheets of the pronuclear envelope. These vesicles appeared to be morphologically similar to the ER vesicles in the ooplasm and were considered to be involved in pronuclear development. Numerous annulate lamellae were seen in the ooplasm as well as in the pronuclei. Ooplasmic crystalloids were also observed. These were thought to represent cytoplasmic yolk. Remnants of the penetrating spermatozoon were found in close relation to one of the pronuclei. The fine structure of the first and second polar body is also described. The nuclear complement of the first polar body consisted of isolated chromosomes, whereas the second polar body contained a membrane-bounded nucleus. In consideration of the possibility that polar body fertilization may take place, these differences in nuclear organization could be of importance. Other recognizable differences between the two polar bodies were presence of dense cortical granules and microvilli in the first polar body, and absence of these structures in the second. These dissimilarities were considered to be related to the organization of the egg cytoplasm at the time of polar body separation.
从一名33岁外科手术患者的输卵管中回收了一枚已受精的卵子，该患者在手术前26小时有过性行为。卵子处于原核期。卵质细胞器主要由线粒体、内质网成分和高尔基体组成。在原核膜的两层之间的空间中发现了小泡。这些小泡在形态上似乎与卵质中的内质网小泡相似，被认为与原核发育有关。在卵质以及原核中都可见到许多环状片层。还观察到卵质晶体。这些被认为代表细胞质卵黄。在与其中一个原核紧密相关的位置发现了穿透精子的残余物。本文还描述了第一极体和第二极体的精细结构。第一极体的核物质由分离的染色体组成，而第二极体含有一个有膜包被的细胞核。考虑到极体受精可能发生的可能性，这些核组织上的差异可能具有重要意义。两个极体之间其他可识别的差异是，第一极体存在密集的皮质颗粒和微绒毛，而第二极体没有这些结构。这些差异被认为与极体分离时卵细胞质的组织有关。
