关于2-氧代醛脱氢酶催化的甲基乙二醛与NADP⁺相关氧化反应的一些观察结果。
Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase.
作者信息
Dunkerton J, James S P
出版信息
Biochem J. 1976 Feb 1;153(2):503-4. doi: 10.1042/bj1530503.
Abstract
In the oxidation of methylglyoxal by 2-oxoaldehyde dehydrogenase, the apparent Km value for NADP+ was about 2.5 times lower than the corresponding Km for NAD+; the apparent Km values for methylglyoxal and for the amine activator L-2-aminopropan-1-ol, with NADP+ as cofactor, were also different from those obtained with NAD+. In the presence of NADP+, the enzyme was not activated by P1, in contrast with the activation of the enzyme when NAD+ was used. The significance of the results is discussed.
摘要
在2-氧代醛脱氢酶催化甲基乙二醛氧化的过程中，NADP⁺的表观Km值比相应的NAD⁺的Km值低约2.5倍；以NADP⁺作为辅因子时，甲基乙二醛和胺激活剂L-2-氨基丙醇-1的表观Km值也与以NAD⁺作为辅因子时得到的值不同。与使用NAD⁺时酶的激活情况相反，在NADP⁺存在时，该酶未被P1激活。文中讨论了这些结果的意义。
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本文引用的文献
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Studies on the inhibition of glyoxalase I by S-substituted glutathiones.S-取代谷胱甘肽对乙二醛酶I的抑制作用研究。
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Glyoxalase inhibitors. A possible approach to anticancer agents.乙二醛酶抑制剂。一种开发抗癌药物的可能途径。
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Alpha-keto aldehyde dehydrogenase, an enzyme that catalyzes the enzymic oxidation of methylglyoxal to pyruvate.α-酮醛脱氢酶，一种催化甲基乙二醛酶促氧化为丙酮酸的酶。
J Biol Chem. 1967 Oct 25;242(20):4603-9.
4
Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines.2-氧代醛脱氢酶的纯化及其对特殊胺类的依赖性。
Biochem J. 1975 Sep;149(3):609-17. doi: 10.1042/bj1490609.
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