成人与新生儿红细胞裂解液中非血红蛋白蛋白质的比较研究。

Comparative studies of non-hemoglobin proteins of adult and newborn red blood cell lysates.

作者信息

Sagisaka K, Iwasa M, Yamashita H, Yamada S

出版信息

Tohoku J Exp Med. 1977 Nov;123(3):287-94. doi: 10.1620/tjem.123.287.

DOI:10.1620/tjem.123.287

Abstract

It has been known that hemolysate is composed of not only hemoglobin but also non-hemoglobin proteins (NHP). NHP isolated effectively from hemolysate of adult and newborn by CM-Sephadex chromatography was investigated by polyacrylamide disc or slab electrophoresis. It was demonstrated on slab electrophoresis that adult NHP is composed of 4 zones (approximately 14 bands) with different mobilities and that adult NHP shows a considerably different pattern from cord NHP. The difference was observed also on the electrophoresis stained for esterase or lactate dehydrogenase. Rabbit antiserum to adult NHP was adsorbed with cord NHP and specific antiserum for adult NHP was prepared. However, antiserum reacting with only cord NHP was not obtained from anti-cord NHP serum. These results suggested that adult specific components with passable antigenicity were present in adult NHP and that cord NHP had similar antigenicity as adult NHP.

摘要

众所周知，溶血产物不仅由血红蛋白组成，还包含非血红蛋白蛋白质（NHP）。通过CM-葡聚糖凝胶色谱法从成人和新生儿的溶血产物中有效分离出的NHP，采用聚丙烯酰胺圆盘或平板电泳进行研究。平板电泳显示，成人NHP由4个区域（约14条带）组成，迁移率不同，且成人NHP与脐带血NHP的模式有很大差异。在酯酶或乳酸脱氢酶染色的电泳中也观察到了这种差异。用脐带血NHP吸附兔抗成人NHP血清，制备了成人NHP的特异性抗血清。然而，从抗脐带血NHP血清中未获得仅与脐带血NHP反应的抗血清。这些结果表明，成人NHP中存在具有相当抗原性的成人特异性成分，且脐带血NHP与成人NHP具有相似的抗原性。