Schubert J, White E R
Science. 1967 Feb 24;155(3765):1000-3. doi: 10.1126/science.155.3765.1000.
Oxygen-carrying capacity of hemocyanin from Limulus and Busycotypus (Busycon) decreases with increasing radiation, giving initial yield values for G(-O(2),) of 1.1 and 1.0, respectively. High radiation doses regenerate this capacity of Busycotypus hemocyanin. These effects are attributed largely to the dual nature of hydrogen peroxide, which, at low concentrations, oxidizes protein-bound copper and at high concentrations, that is, at high doses, reduces oxidized copper. The ability of hemocyanin to decompose hydrogen peroxide is relatively unaffected by irradiation, which suggests that copper atoms at the active sites are not all equivalent. The catalase-like activity of Busycotypus hemocyanin can be simulated by amino acid chelates of copper, including arginine, histidine, and glycine.
鲎和Busycotypus（Busycon）血蓝蛋白的携氧能力随辐射剂量增加而降低，其G(-O(2),)的初始产率值分别为1.1和1.0。高辐射剂量可使Busycotypus血蓝蛋白的这种能力再生。这些效应在很大程度上归因于过氧化氢的双重性质，即在低浓度时，它会氧化与蛋白质结合的铜，而在高浓度时，也就是高剂量时，它会还原被氧化的铜。血蓝蛋白分解过氧化氢的能力相对不受辐射影响，这表明活性位点上的铜原子并非全部等同。Busycotypus血蓝蛋白的过氧化氢酶样活性可以被铜的氨基酸螯合物模拟，包括精氨酸、组氨酸和甘氨酸。
