Drew S I, Carter B M, Guidera D P, Terasaki P I
Transplant Proc. 1977 Dec;9(4):1887-94.
Optimum serologic reactivity is observed if papain-treated granulocytes are reacted with cytotoxic antibody at low (5 degrees C) rather than warm (22 degrees C) precomplement incubation temperatures. Favorable in vitro conditions have allowed the identification of cytotoxic granulocyte antibodies in approximately 12% of nonimmunized normal males and females. Furthermore, the incidence of granulocytotoxic antisera in a group of alloimmunized patients did not exceed that observed in the normal population. In two cases cited, a normal male and a patient with pathologic neutropenia, cytotoxic antibodies against allogeneic granulocytes were autoreactive against the autologous cells of the serum producer. In the latter subject, an inverse association was demonstrated between the presence of autoantibody and the circulating neutrophil count. The incidence of granulocytotoxins in various diseases has been given and appears raised in systemic lupus erythematosus and asthma.
如果用木瓜蛋白酶处理过的粒细胞在低（5摄氏度）而非温（22摄氏度）的补体预孵育温度下与细胞毒性抗体反应，可观察到最佳血清学反应性。有利的体外条件已使得在大约12%的未免疫正常男性和女性中鉴定出细胞毒性粒细胞抗体。此外，一组同种免疫患者中粒细胞毒性抗血清的发生率并未超过正常人群中观察到的发生率。在引用的两个案例中，一名正常男性和一名患有病理性中性粒细胞减少症的患者，针对同种异体粒细胞的细胞毒性抗体对血清产生者的自体细胞具有自身反应性。在后者中，自身抗体的存在与循环中性粒细胞计数之间呈负相关。已给出各种疾病中粒细胞毒素的发生率，在系统性红斑狼疮和哮喘中似乎有所升高。
