Chebanu F A, Kushch A A, Zelenin A V
Vopr Onkol. 1977;23(12):64-6.
Cytological preparations of normal cervical epithelium and in different forms of the epithelial dysplasia and in early cervical cancer were studied in 8 healthy females and in 35 patients. To determine the content of DNA in these cells a fluorescent variant of the Feulgen reaction was employed, using the national drug rivanol as fluorochromium in the Shiff reagent. The amount of DNA in cell nuclei of the normal epithelium and in moderate dysplasia is shown to correspond to diploid, while in marked dysplasia the content of DNA varies from di-to tetraploid values. But there are some cases when in marked dysplasia the DNA content in cells is diploid, i. e. it is characteristic of moderate dysplasia, or considerably exceeds tetraploid level, and it is in such cases that histological assays reveal early forms of cancer. Invasive cancer is charcterized by a sharp increase in the DNA content in most cells and by a considerable number of polyploid cells. Fluorescence-microscopy may serve as a valuable diagnostic adjunct to recognize early forms of cervical cancer and be used clinically.
对8名健康女性和35名患者的正常宫颈上皮、不同形式的上皮发育异常及早期宫颈癌的细胞学标本进行了研究。为测定这些细胞中的DNA含量，采用了Feulgen反应的荧光变体，使用国产药物利凡诺作为希夫试剂中的荧光染色剂。结果显示，正常上皮细胞核及中度发育异常细胞核中的DNA含量与二倍体相符，而在重度发育异常中，DNA含量在二倍体至四倍体之间变化。但在某些重度发育异常病例中，细胞中的DNA含量为二倍体，即具有中度发育异常的特征，或显著超过四倍体水平，正是在这些病例中，组织学检测发现了早期癌症形式。浸润性癌的特征是大多数细胞中的DNA含量急剧增加，且有相当数量的多倍体细胞。荧光显微镜检查可作为识别早期宫颈癌形式的有价值的诊断辅助手段，并可用于临床。
