Itakura Y, Sasano F, Date F, Kato K, Sekine H, Mori S, Nagura H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Anticancer Res. 1996 Jan-Feb;16(1):201-8.
Ki67 expression, S-phase fraction, p53 immunoreactivity and DNA content were examined in morphologically normal mucosa and squamous dysplasia of both cancerous and non-cancerous human oesophagi in order to understand possible early events in the development of esophageal squamous cell carcinoma. 103 different foci from cancerous esophagi including 17 non-pathological epithelium, 10 mild, 17 moderate and 15 severe dysplasia, 14 intraepithelial carcinomas and 30 invasive squamous cell carcinomas were examined. Also studied were 57 biopsy specimens from cancer-free individuals, including 12 normal epithelia, 15 oesophagitis, and 16 mild, 11 moderate and 3 severe dysplasia. Areas of squamous dysplasia from both cancer-free and cancerous oesophagi were morphologically indistinguishable and both demonstrated increased cellular proliferation compared to normal or non-pathological epithelia. However, squamous dysplasia in cancerous oesophagi demonstrated significantly larger ki67 labelling indices and smaller S-phase fractions than dysplasia in cancer-free patients. Squamous dysplasia in cancerous and non-cancerous oesophagi demonstrated an non-diploid DNA histogram in 67.9% and 43.3% respectively. However, dysplasia from cancer-free individuals demonstrated a non-diploid pattern with one or more peaks (Type I non-diploid histogram) and that from oesophageal cancer patients predominantly exhibited non-diploid histograms without any distinctive peaks (Type II non-diploid histogram). Significant differences in the frequency of p53 positive foci were observed between dysplasia of cancer-free (23.3%) and cancerous (56.8%) oesophagi. IN cancerous oesophagi, dysplasia associated with Type II non-diploid histograms had a significantly larger number of p53-positive foci than those with diploid histograms or Type I non-diploid histograms. These results indicated that the biological features of squamous dysplasia were different between cancerous and non-cancerous human oesophagi despite indistinguishable morphological features. In addition, the combination of p53 immuno-histochemistry and DNA ploidy analysis may contribute to identify possible high-risk squamous dysplasia of the oesophagus.
检测了人癌性和非癌性食管形态学正常黏膜及鳞状上皮发育异常中的Ki67表达、S期细胞比例、p53免疫反应性及DNA含量，以了解食管鳞状细胞癌发生发展过程中可能的早期事件。检测了来自癌性食管的103个不同病灶，包括17个非病理性上皮、10个轻度、17个中度和15个重度发育异常、14个上皮内癌和30个浸润性鳞状细胞癌。还研究了57例来自无癌个体的活检标本，包括12个正常上皮、15个食管炎，以及16个轻度、11个中度和3个重度发育异常。来自无癌和癌性食管的鳞状上皮发育异常区域在形态上无法区分，与正常或非病理性上皮相比，两者均显示细胞增殖增加。然而，癌性食管中的鳞状上皮发育异常显示出比无癌患者的发育异常更大的Ki67标记指数和更小的S期细胞比例。癌性和非癌性食管中的鳞状上皮发育异常分别有67.9%和43.3%表现为非二倍体DNA直方图。然而，来自无癌个体的发育异常表现为具有一个或多个峰的非二倍体模式（I型非二倍体直方图），而来自食管癌患者的发育异常主要表现为无任何明显峰的非二倍体直方图（II型非二倍体直方图）。在无癌（23.3%）和癌性（56.8%）食管的发育异常之间，观察到p53阳性病灶频率存在显著差异。在癌性食管中，与II型非二倍体直方图相关的发育异常比具有二倍体直方图或I型非二倍体直方图的发育异常有显著更多的p53阳性病灶。这些结果表明，尽管形态特征无法区分，但人癌性和非癌性食管中鳞状上皮发育异常的生物学特征不同。此外，p53免疫组织化学和DNA倍体分析的联合应用可能有助于识别食管可能的高危鳞状上皮发育异常。
