Lieberman E M, Perkins M S, Tomita T, Wright E B
Science. 1967 Apr 14;156(3772):240-2. doi: 10.1126/science.156.3772.240.
The bioelectrical characteristics of a crab nerve fiber subjected to solutions containing the impermeant anions ferrocyanide, glutamate, or ethanolsulfate substituted for chloride include prolonged slow depolarizations that elicit prolonged trains of impulses sulperimposed on the plateau portion. Propionate and nitrate depress repetitive firing while thiocyancite has only a slighit effect. Nerves treated with ferrocyanide lose potassium and gain sodium, which fact may account for their depolarization. On the other hand, studies with interference microscopy reveal shift by ferrocyanide in the interference fringe pattern of the sheath material; this suggests a reorientation of fixed charges in the protein layers by direct action of the anion on these layers. This may also account for the electrical manifestations observed.
将蟹神经纤维置于含有不可渗透阴离子（亚铁氰化物、谷氨酸盐或乙醇硫酸盐，以取代氯离子）的溶液中时，其生物电特性包括长时间的缓慢去极化，这种去极化会引发叠加在平台期的长时间冲动序列。丙酸盐和硝酸盐会抑制重复放电，而硫氰酸盐的影响较小。用亚铁氰化物处理过的神经会失去钾并获得钠，这一事实可能解释了它们的去极化现象。另一方面，干涉显微镜研究显示，亚铁氰化物会使鞘材料的干涉条纹图案发生偏移；这表明阴离子直接作用于蛋白质层，使其中的固定电荷重新定向。这也可能解释所观察到的电现象。
