过硫酸铵在圆盘电泳中产生的假象。
Artifact produced in disc electrophoresis by ammonium persulfate.
作者信息
Brewer J M
出版信息
Science. 1967 Apr 14;156(3772):256-7. doi: 10.1126/science.156.3772.256.
PMID:6021045
Abstract
Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.
摘要
过硫酸铵是丙烯酰胺凝胶常用的聚合剂，它能使酵母烯醇化酶失活，并在含有8M尿素的凝胶圆盘电泳过程中导致电泳异质性增加。使用核黄素和光照进行聚合反应或使用巯基乙酸盐去除过硫酸铵是可行的替代方法。
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