通过自由流动电泳分离胶原蛋白的α链。
The separation of the alpha-chains of collagen by free-flow electrophoresis.
作者信息
Francois C J, Glimcher M J
出版信息
Biochem J. 1967 Jan;102(1):148-52. doi: 10.1042/bj1020148.
Abstract
- The separation of the alpha-components of codfish-skin and calf-skin collagen from the higher-molecular-weight components was accomplished by gel filtration with Bio-Gel P-300 resin in 5m-lithium chloride and at pH7.4. 2. From this fraction three distinct alpha-components, corresponding to the alpha1-, alpha2- and alpha3- chains of collagen, were isolated by free-flow electrophoresis in 6m-urea at 4 degrees . At the temperatures and pH values used there was no detectable degradation of the alpha-chains.
摘要
- 通过在5M氯化锂和pH7.4条件下使用Bio-Gel P-300树脂进行凝胶过滤，将鳕鱼皮和小牛皮胶原蛋白的α组分与高分子量组分分离。2. 从该级分中，通过在4℃下于6M尿素中进行自由流动电泳，分离出对应于胶原蛋白α1-、α2-和α3-链的三种不同的α组分。在所使用的温度和pH值下，α链没有可检测到的降解。
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