亚甲蓝氧化产生的污渍成分。

The composition of stains produced by the oxidation of Methylene Blue.

作者信息

Marshall P N

出版信息

Histochem J. 1976 Jul;8(4):431-42. doi: 10.1007/BF01003831.

Abstract

The composition of some stains produced by the oxidation of Methylene Blue has been studied by thin-layer chromatography. Various named methods for the production of Polychrome Methylene Blue, Azure A, Azure B, Azure C and Methylene Violet Bernthsen have been found to give complex mixtures of varying proportions of up to eleven dyes. Ten of these, namely Methylene Blue, Azure B, Azure A, sym-Dimethylthionine, Azure C, Thionine, Methylene Violet Bernthsen, Methyl Thionoline, Thionoline and Thionol, have been identified by their visible absorption spectra. The remaining dye could not be identified. When used on a laboratory scale, these methods give stains of constant composition independent of the batch of Methylene Blue. Stain composition as revealed in the present study has been compared with that previously indicated by other, less effective, analytical techniques. Reasons are presented why the latter give equivocal results.

摘要

通过薄层色谱法研究了亚甲蓝氧化产生的一些染色剂的成分。已发现各种命名的制备多色亚甲蓝、天青A、天青B、天青C和亚甲紫伯恩森的方法会产生多达11种染料的不同比例的复杂混合物。其中十种，即亚甲蓝、天青B、天青A、对称二甲基硫堇、天青C、硫堇、亚甲紫伯恩森、甲基硫代啉、硫代啉和硫醇，已通过其可见吸收光谱进行了鉴定。其余的染料无法鉴定。当在实验室规模上使用时，这些方法产生的染色剂成分恒定，与亚甲蓝的批次无关。本研究中揭示的染色剂成分已与其他效果较差的分析技术先前指出的成分进行了比较。阐述了后者产生含糊结果的原因。