Bork K
Arch Dermatol Res (1975). 1977 Dec 27;260(3):217-26. doi: 10.1007/BF00561416.
The physical forces operative in the fluid migration from the interstitial spaces into the blister cleft have not been directly measured until now. The colloid osmotic pressure was determined in suction blister fluid after mild suction blister production by a modified "Dermovac" and in blister fluid of patients with dermatitis herpetiformis, bullous allergic contact dermatitis and pemphrigus vulgaris and in the sera of healthy persons. The colloid osmotic pressure was measured by means of a recently developed osmometer with a semipermeable membrane between two chambers, one of them filled with Ringer solution, the other with the blister fluid sample. The negative pressure in the first chamber was determined. The colloid osmotic pressure of suction blister fluid averages approximately 7 cm H2O, the values reach about 20 cm H2O in bullous diseases and about 38 cm H2O in the normal sera. The blister fluid colloid osmotic pressure has to rise to about 15 cm H2O or more to cause the fluid transport from the interstitial spaces of the surrounding tissue into the blister because of the negative interstitial fluid pressure and the colloid osmotic pressure of the interstitial fluid. Otherwise the blister fluid is reabsorbed back into the interstitial spaces.
迄今为止，尚未直接测量从间质间隙进入水疱裂隙的液体迁移过程中起作用的物理力。通过改良的“Dermovac”产生轻度抽吸水疱后，测定抽吸水疱液中的胶体渗透压，同时测定疱疹样皮炎、大疱性过敏性接触性皮炎和寻常型天疱疮患者水疱液以及健康人血清中的胶体渗透压。胶体渗透压通过一种最近开发的渗透计进行测量，该渗透计在两个腔室之间有一个半透膜，其中一个腔室充满林格溶液，另一个腔室充满水疱液样本。测定第一个腔室中的负压。抽吸水疱液的胶体渗透压平均约为7 cmH₂O，在大疱性疾病中该值可达约20 cmH₂O，在正常血清中约为38 cmH₂O。由于间质液负压和间质液胶体渗透压的存在，水疱液胶体渗透压必须升至约15 cmH₂O或更高，才能使液体从周围组织的间质间隙转运至水疱中。否则，水疱液会被重新吸收回间质间隙。
