Bork K
J Invest Dermatol. 1978 Sep;71(3):209-12. doi: 10.1111/1523-1747.ep12547271.
The physical forces operative in the fluid migration from the interstitial spaces into the blister cleft have not been directly measured until now. The colloid osmotic pressure and the total osmolality were determined in suction blister fluid after mild suction blister production by a modified "Dermovac" and in blister fluid of patients with dermatitis herpetiformis, bullous allergic contact dermatitis and pemphigus vulgaris and in the sera of healthy persons. The colloid osmotic pressure was measured by means of a recently developed osmometer with a semipermeable membrane between 2 chambers, one of them filled with Ringer solution, the other with the blister fluid or serum sample. The negative pressure in the first chamber was determined. The colloid osmotic pressure of suction blister fluid averages approximately 7 cm H2O, the values reach about 20 cm H2O in bullous diseases and about 38 cm H2O in the normal sera. The blister fluid colloid osmotic pressure has to rise to about 15 cm H2O or more to cause the fluid transport from the interstitial spaces of the surrounding tissue into the blister because of the negative interstitial fluid pressure and the colloid osmotic pressure of the interstitial fluid. Otherwise the blister fluid is reabsorbed back into the interstitial spaces. The total osmolality does not differ in the serum and in the blister fluid. It does not seem to be etiologically connected with the fluid transport into the rising blister.
迄今为止，尚未直接测量过促使液体从组织间隙迁移至水疱裂隙的物理力。通过改良的“Dermovac”产生轻度抽吸水疱后，测定了抽吸水疱液中的胶体渗透压和总渗透压，还测定了疱疹样皮炎、大疱性过敏性接触性皮炎和寻常型天疱疮患者水疱液以及健康人血清中的胶体渗透压和总渗透压。胶体渗透压通过一种最近开发的渗透计进行测量，该渗透计在两个腔室之间有一个半透膜，其中一个腔室充满林格溶液，另一个腔室充满水疱液或血清样本。测定第一个腔室中的负压。抽吸水疱液的胶体渗透压平均约为7 cmH₂O，在大疱性疾病中该值可达约20 cmH₂O，在正常血清中约为38 cmH₂O。由于组织间隙液的负压和胶体渗透压，水疱液的胶体渗透压必须升至约15 cmH₂O或更高，才能促使液体从周围组织的组织间隙转运至水疱中。否则，水疱液会被重新吸收回组织间隙。血清和水疱液中的总渗透压没有差异。它似乎与液体转运至正在形成的水疱在病因学上没有关联。
