一种大规模分离肾上腺嗜铬颗粒的简单方法。
A simple method for the isolation of adrenal chromaffin granules on a large scale.
作者信息
Smith A D, Winkler H
出版信息
Biochem J. 1967 May;103(2):480-2. doi: 10.1042/bj1030480.
Abstract
Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.
摘要
通过使用角头转子，将大颗粒部分分层在1.6M蔗糖溶液上进行超速离心，可从牛、猪、马或大鼠肾上腺髓质的匀浆中获得高度纯化的嗜铬颗粒。嗜铬颗粒以粉红色沉淀物的形式获得，仅轻微受到线粒体和溶酶体的污染。
相似文献
1
A simple method for the isolation of adrenal chromaffin granules on a large scale.
Biochem J. 1967 May;103(2):480-2. doi: 10.1042/bj1030480.
2
Some chemical and physical properties of the soluble protein fraction of bovine adrenal chromaffin granules.
Mol Pharmacol. 1966 Jul;2(4):298-310.
3
Uptake of nucleotides and catecholamines by chromaffin granules from pig and horse adrenal medulla.
J Neurochem. 1980 Jul;35(1):270-2. doi: 10.1111/j.1471-4159.1980.tb12516.x.
4
[Preparation of highly purified chromaffin granules from ox adrenal medullae].
Biochem Pharmacol. 1968 Jan;17(1):87-91. doi: 10.1016/0006-2952(68)90161-5.
5
A new and simple method for isolation of adrenal chromaffin granules by means of an isotonic density gradient.
Anal Biochem. 1970 Apr;34(2):403-12. doi: 10.1016/0003-2697(70)90125-9.
6
THE ADENOSINE-TRIPHOSPHATASE ACTIVITY OF ADRENAL CHROMAFFIN GRANULES.
Biochem J. 1965 May;95(2):490-6. doi: 10.1042/bj0950490.
7
Studies on the proteins from chromaffin granules of ox, horse and pig.
Nature. 1966 Aug 27;211(5052):982-3. doi: 10.1038/211982a0.
8
An analysis of nucleotides and catecholamines in bovine medullary granules by anion exchange high pressure liquid chromatography and fluorescence. Evidence that most of the catecholamines in chromaffin granules are stored without associated ATP.
Pharmacology. 1977;15(5):377-91. doi: 10.1159/000136714.
9
Is lysolecithin an in vivo constituent of chromaffin granules?
J Neurochem. 1983 Nov;41(5):1480-3. doi: 10.1111/j.1471-4159.1983.tb00848.x.
10
Comparison of spontaneous loss of catecholamines and ATP in vitro from isolated bovine adrenomedullary, vesicular gland, vas deferens and splenic nerve granules.
J Neurochem. 1966 Nov;13(11):1213-6. doi: 10.1111/j.1471-4159.1966.tb04279.x.
引用本文的文献
1
A complex role of chromogranin A and its peptides in inflammation, autoimmunity, and infections.
Front Immunol. 2025 Apr 30;16:1567874. doi: 10.3389/fimmu.2025.1567874. eCollection 2025.
2
Isolation and Purification of Chromaffin Granules from Adrenal Glands and Cultured Neuroendocrine Cells.
Methods Mol Biol. 2023;2565:283-296. doi: 10.1007/978-1-0716-2671-9_19.
3
Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies.
Sci Rep. 2020 May 5;10(1):7540. doi: 10.1038/s41598-020-64486-3.
4
Phosphopeptidomics Reveals Differential Phosphorylation States and Novel SxE Phosphosite Motifs of Neuropeptides in Dense Core Secretory Vesicles.
J Am Soc Mass Spectrom. 2018 May;29(5):935-947. doi: 10.1007/s13361-018-1915-0. Epub 2018 Mar 19.
5
MicroRNA exocytosis by large dense-core vesicle fusion.
Sci Rep. 2017 Mar 30;7:45661. doi: 10.1038/srep45661.
6
Profiles of secreted neuropeptides and catecholamines illustrate similarities and differences in response to stimulation by distinct secretagogues.
Mol Cell Neurosci. 2015 Sep;68:177-85. doi: 10.1016/j.mcn.2015.06.008. Epub 2015 Jun 16.
7
Pyroglutamate-amyloid-β and glutaminyl cyclase are colocalized with amyloid-β in secretory vesicles and undergo activity-dependent, regulated secretion.
Neurodegener Dis. 2014;14(2):85-97. doi: 10.1159/000358430. Epub 2014 Jun 18.
8
Beta-amyloid peptides undergo regulated co-secretion with neuropeptide and catecholamine neurotransmitters.
Peptides. 2013 Aug;46:126-35. doi: 10.1016/j.peptides.2013.04.020. Epub 2013 Jun 6.
9
Myosin VI small insert isoform maintains exocytosis by tethering secretory granules to the cortical actin.
J Cell Biol. 2013 Feb 4;200(3):301-20. doi: 10.1083/jcb.201204092.
10
Controlling synaptotagmin activity by electrostatic screening.
Nat Struct Mol Biol. 2012 Oct;19(10):991-7. doi: 10.1038/nsmb.2375. Epub 2012 Sep 2.
本文引用的文献
1
Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids.
Biochim Biophys Acta. 1950 Jan;4(1-3):211-4. doi: 10.1016/0006-3002(50)90026-6.
2
THE ADENOSINE-TRIPHOSPHATASE ACTIVITY OF ADRENAL CHROMAFFIN GRANULES.
Biochem J. 1965 May;95(2):490-6. doi: 10.1042/bj0950490.
3
Improved technique for the fluorimetric estimation of catecholamines.
Acta Physiol Scand. 1961 Apr;51:348-55. doi: 10.1111/j.1748-1716.1961.tb02128.x.
4
The distribution of the catechol amines in homogenates of the bovine adrenal medulla.
J Physiol. 1958 Apr 3;141(1):183-92. doi: 10.1113/jphysiol.1958.sp005966.
5
Mitochondrial enzymes and chromaffin granules.
J Physiol. 1957 Dec 3;139(2):316-22. doi: 10.1113/jphysiol.1957.sp005893.
6
Observations on the distribution of catechol amines and adenosinetriphosphate in the bovine adrenal medulla.
J Physiol. 1956 Sep 27;133(3):548-57. doi: 10.1113/jphysiol.1956.sp005607.
7
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
8
Determination of ATP and related compounds: firefly luminescence and other methods.
Methods Biochem Anal. 1954;1:341-56. doi: 10.1002/9780470110171.ch13.
9
The localization of lysosomal enzymes in chromaffin tissue.
J Physiol. 1966 Mar;183(1):179-88. doi: 10.1113/jphysiol.1966.sp007859.
10
Purification and properties of an acidic protein from chromaffin granules of bovine adrenal medulla.
Biochem J. 1967 May;103(2):483-92. doi: 10.1042/bj1030483.
Zotero 插件