Goodman F R, Gardiner T H
Arch Int Pharmacodyn Ther. 1977 Nov;230(1):31-41.
The distribution and movements of 45Ca were investigated in lung slice preparations obtained from rats. The accumulation of 45Ca was measured in either 5.0 mM Ca2+, 0.5 mM Ca2+ or 0-Ca solutions. The 45Ca tissue to medium ratio was greatest in the 0-Ca solution; the presence of either 5.0 mM Ca2+ or 0.5 mM Ca2+ reduced this ratio 8- and 5-fold, respectively. Efflux of 45Ca indicated that there are at least two washout components. Exposure to either La3+ or Sr2+ (0.5 mM) during the washout resulted in a transient increase in 45Ca efflux (Sr2+ greater than La3+), whereas 0 degrees C slowed the loss of 45Ca. Uptake of 45Ca was increased by the metabolic inhibitor iodoacetic acid and inhibited in the presence of either La3+ or Sr2+. These findings indicate that the 45Ca accumulated within the lung is not readily depleted and a portion appears to accumulate at intracellular or less superficial sites or stores.
