Mostafa M A, El-Aassar S T
Arch Exp Veterinarmed. 1977;31(4):567-74.
New methods for the microdetermination of ascorbic acid are described. The 3 proposed methods based on the fact that N-chlorosuccinimide in acid, alkaline or neutral medium readily oxidizes ascorbic acid. The mechanism of the reaction is discussed. The proposed methods are simple rapid, and sufficiently accurate to determine concentrations as low as 10 micrograms of ascorbic acid. The experimental error does not exceed +/- 2%. The methods were also used for the determination of ascorbic acid in such biological fluids as bloody, urine or edible fruits with an error not exceeding +/- 5%.
本文描述了微量测定抗坏血酸的新方法。所提出的3种方法基于这样一个事实：在酸性、碱性或中性介质中的N-氯代琥珀酰亚胺能轻易氧化抗坏血酸。讨论了反应机理。所提出的方法简单、快速，并且足够准确，能够测定低至10微克抗坏血酸的浓度。实验误差不超过±2%。这些方法还用于测定血液、尿液或可食用水果等生物流体中的抗坏血酸，误差不超过±5%。
