猫大脑皮层灌流液中氨基酸的1-二甲基氨基萘-5-磺酰基衍生物的薄层色谱分析。
Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex.
作者信息
Crowshaw K, Jessup S J, Ramwell P W
出版信息
Biochem J. 1967 Apr;103(1):79-85. doi: 10.1042/bj1030079.
Abstract
- Five new solvent systems are reported for the separation of 1-dimethylaminonaphthalene-5-sulphonylamino acids by thin-layer chromatography on silica gel. After two-dimensional chromatography with a suitable pair of these solvent systems, most of the 1-dimethylaminonaphthalene-5-sulphonyl derivatives were completely separated and could be located by their intense yellow fluorescence when viewed under u.v. light. 2. These techniques have been used to identify 21 amino acids present in superfusates of cat cerebral cortex, plasma and cerebrospinal fluid. 3. A method for the semiquantitative estimation of amino acids in biological fluids is described in which the fluorescent intensity of their separated 1-dimethylaminonaphthalene-5-sulphonyl derivatives was measured.
摘要
- 报道了五种新的溶剂系统，用于在硅胶上通过薄层色谱法分离1-二甲基氨基萘-5-磺酰基氨基酸。在用这些溶剂系统中的合适组合进行二维色谱分析后，大多数1-二甲基氨基萘-5-磺酰基衍生物被完全分离，并且在紫外光下观察时可通过其强烈的黄色荧光进行定位。2. 这些技术已用于鉴定猫脑皮质、血浆和脑脊液超滤液中存在的21种氨基酸。3. 描述了一种用于生物流体中氨基酸半定量估计的方法，其中测量了其分离的1-二甲基氨基萘-5-磺酰基衍生物的荧光强度。
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Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex.猫大脑皮层灌流液中氨基酸的1-二甲基氨基萘-5-磺酰基衍生物的薄层色谱分析。
Biochem J. 1967 Apr;103(1):79-85. doi: 10.1042/bj1030079.
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本文引用的文献
1
The formation of 1-dimethylaminonaphthalene-5-sulphonamide during the preparation of 1-dimethylaminonaphthalene-5-sulphonylamino acids.在制备1-二甲基氨基萘-5-磺酰基氨基酸过程中1-二甲基氨基萘-5-磺酰胺的形成。
Biochem J. 1965 Nov;97(2):607-8. doi: 10.1042/bj0970607.
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AMINO ACID RELEASE FROM THE CEREBRAL CORTEX DURING SPREADING DEPRESSION AND ASPHYXIATION.扩散性抑制和窒息期间大脑皮质氨基酸的释放
J Neurochem. 1965 May;12:431-9. doi: 10.1111/j.1471-4159.1965.tb04243.x.
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AMINO ACIDS RELEASED FROM THE CEREBRAL CORTEX IN RELATION TO ITS STATE OF ACTIVATION.从大脑皮层释放的氨基酸与其激活状态的关系。
Science. 1965 Mar 19;147(3664):1448-9. doi: 10.1126/science.147.3664.1448.
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Studies on the free amino acids and related compounds in the tissues of the cat.猫组织中游离氨基酸及相关化合物的研究。
J Biol Chem. 1954 Dec;211(2):927-39.
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Thin-layer chromatographic separation of DNS-amino acids.DNS-氨基酸的薄层色谱分离
Anal Biochem. 1966 Mar;14(3):429-33. doi: 10.1016/0003-2697(66)90285-5.
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J Chromatogr. 1966 Jan;21(1):135-7. doi: 10.1016/s0021-9673(01)91275-0.
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