Shamgar F A, Collins F D
Aust J Exp Biol Med Sci. 1977 Aug;55(4):371-8. doi: 10.1038/icb.1977.33.
The contribution of phosphatidylethanolamine methylation to phosphatidylcholine biosynthesis in rat muscle was investigated by studying the incorporation of [2-3H] ethanolamine. The specific radioactivities of individual molecular species of muscle phosphoglycerides were measured by a combination of argentation thin-layer chromatography and countercurrent distribution. The specific radioactivity of phosphatidylethanolamine was approximately one thousand times that of phosphatidylcholine. Amongst individual phosphatidylethanolamines, hexaenoic species possessed the highest specific radioactivities and tetraenoic the lowest. Because of the very low incorporation into phosphatidylcholine, the specific radioactivities of combined rather than of individual fractions were measured. The results indicate that the contribution of phosphatidylethanolamine methylation to the overall biosynthesis of phosphatidylcholine in muscle is of minor importance.
通过研究[2-³H]乙醇胺的掺入情况，对大鼠肌肉中磷脂酰乙醇胺甲基化在磷脂酰胆碱生物合成中的作用进行了研究。采用银化薄层色谱法和逆流分配法相结合的方式，测定了肌肉磷酸甘油酯各个分子种类的比放射性。磷脂酰乙醇胺的比放射性约为磷脂酰胆碱的一千倍。在各个磷脂酰乙醇胺中，六烯酸种类的比放射性最高，四烯酸种类的最低。由于掺入磷脂酰胆碱的量非常低，因此测定的是混合部分而非各个部分的比放射性。结果表明，磷脂酰乙醇胺甲基化对肌肉中磷脂酰胆碱整体生物合成的作用较小。
