Krulwich T A, Ensign J C, Tipper D J, Strominger J L
J Bacteriol. 1967 Sep;94(3):734-40. doi: 10.1128/jb.94.3.734-740.1967.
Cell walls of Arthrobacter crystallopoietes were prepared from cells grown as spheres and from peptone- and succinate-induced rod stage cells. Undegraded polysaccharide backbones of the peptidoglycans were isolated from myxobacter AL-1 protease digests by ECTEOLA cellulose and Sephadex G-50 chromatography. The polysaccharide backbones of the sphere cell wall peptidoglycan are heterogeneous in their size, and average less than 40 hexosamines per chain. Those of the rod cell walls are homogeneous in size and average 114 to 135 hexosamines per chain.
节杆菌结晶生成菌的细胞壁是从以球状生长的细胞以及由蛋白胨和琥珀酸盐诱导的杆状阶段细胞制备而来的。肽聚糖未降解的多糖主链通过ECTEOLA纤维素和葡聚糖G - 50色谱从粘细菌AL - 1蛋白酶消化物中分离出来。球状细胞壁肽聚糖的多糖主链大小不均一,每条链平均少于40个己糖胺。杆状细胞壁的多糖主链大小均一,每条链平均有114至135个己糖胺。