大脑皮层切片研究。以葡萄糖或丙酮酸为底物时，各种抑制剂对钾离子和氨基酸保留的影响。

Studies on brain-cortex slices. The influence of various inhibitors on the retention of potassium ions and amino acids with glucose or pyruvate as substrate.

Cremer J E

Biochem J. 1967 Jul;104(1):223-8. doi: 10.1042/bj1040223.

Abstract

The K(+) content of brain-cortex slices incubated in a Krebs-Ringer phosphate medium was the same whether the substrate was glucose (11mm) or pyruvate (11mm). 2. 2,4-Dinitrophenol (30mum) and ouabain (83mum) lowered the K(+) content of brain-cortex slices by approx. 40% with either glucose or pyruvate as the substrate. Triethyltin (1.3mum) lowered the K(+) content by 73% with glucose as substrate, but by only 18% when the substrate was pyruvate. 3. Quantitative estimations of six individual amino acids were made on brain-cortex slices and on the incubation medium after 75min. at 37 degrees . Some differences were found depending on whether the substrate was glucose or pyruvate. The effects of 2,4-dinitrophenol, oligomycin, triethyltin and ouabain were studied. Triethyltin caused a marked increase in the medium of each amino acid when the substrate was glucose, but not when it was pyruvate. Ouabain caused a similar increase with either glucose or pyruvate as substrate. 4. The uptake of triethyltin by brain-cortex slices was measured by using radioactive triethyl[(113)Sn]tin chloride. Various experimental conditions did not alter the uptake; these included adding glucose or pyruvate as a substrate. 5. From the author's own results and those of others it is suggested that pyruvate is an unsuitable substrate to use for the study of regulatory factors of endogenous pyruvate metabolism in brain-cortex slices. Glucose is preferable.

摘要

在磷酸 Krebs - Ringer 培养基中孵育的大脑皮层切片，无论底物是葡萄糖（11 毫摩尔）还是丙酮酸（11 毫摩尔），其钾离子含量均相同。2. 2,4 - 二硝基苯酚（30 微摩尔）和哇巴因（83 微摩尔）使大脑皮层切片的钾离子含量降低约 40%，以葡萄糖或丙酮酸作为底物时均如此。三乙锡（1.3 微摩尔）以葡萄糖为底物时使钾离子含量降低 73%，但以丙酮酸为底物时仅降低 18%。3. 在 37 摄氏度孵育 75 分钟后，对大脑皮层切片和孵育培养基中的六种单个氨基酸进行了定量测定。根据底物是葡萄糖还是丙酮酸，发现了一些差异。研究了 2,4 - 二硝基苯酚、寡霉素、三乙锡和哇巴因的作用。当底物是葡萄糖时，三乙锡使每种氨基酸在培养基中的含量显著增加，但当底物是丙酮酸时则不然。以葡萄糖或丙酮酸为底物时，哇巴因都会引起类似的增加。4. 通过使用放射性三乙基[(113)Sn]氯化锡来测量大脑皮层切片对三乙锡的摄取。各种实验条件均未改变摄取量；这些条件包括添加葡萄糖或丙酮酸作为底物。5. 根据作者自己的结果以及其他人的结果表明，丙酮酸不是用于研究大脑皮层切片内源性丙酮酸代谢调节因子的合适底物。葡萄糖更可取。