渗透“休克”对大鼠肝脏线粒体肿胀模式及呼吸控制的影响。
The effect of osmotic 'shock' on the swelling pattern and respiratory control of rat-liver mitochondria.
作者信息
Streichman S, Avi-Dor Y
出版信息
Biochem J. 1967 Jul;104(1):71-7. doi: 10.1042/bj1040071.
Abstract
- Rat-liver mitochondria suspended in 0.25m-sucrose were exposed for a few seconds to strongly hypo-osmotic conditions, and then the osmolarity of the medium was raised again to 0.25 with the aid of tris chloride (osmotic ;shock'). 2. Mitochondria after hypo-osmotic pretreatment lost their capacity for slow energy-dependent swelling in iso-osmotic tris buffer and showed no respiratory control. 3. Swelling could be induced in the ;shocked' mitochondria by ATP but not by addition of respiratory substrates. 4. It was shown that cytochrome c is lost from ;shocked' mitochondria when they come into contact with the tris buffer present in the assay medium, and that the changes observed in the pattern of swelling, as well as in respiratory control, are directly connected with this loss of cytochrome c. 5. The results of the investigation are discussed with regard to the role of cytochrome c in swelling and respiratory control.
摘要
- 将悬浮于0.25m蔗糖溶液中的大鼠肝脏线粒体暴露于强低渗条件下几秒，然后借助三氯化物将培养基的渗透压再次提高至0.25（渗透压“休克”）。2. 经低渗预处理后的线粒体在等渗三羟甲基氨基甲烷缓冲液中丧失了缓慢的能量依赖性肿胀能力，且无呼吸控制。3. ATP可诱导“休克”后的线粒体肿胀，但添加呼吸底物则不能。4. 结果表明，“休克”后的线粒体与测定培养基中的三羟甲基氨基甲烷缓冲液接触时会丢失细胞色素c，并且在肿胀模式以及呼吸控制中观察到的变化与细胞色素c的这种丢失直接相关。5. 就细胞色素c在肿胀和呼吸控制中的作用对研究结果进行了讨论。
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