Korn E D
J Cell Biol. 1967 Aug;34(2):627-38. doi: 10.1083/jcb.34.2.627.
Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0 degrees . As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid.
将油酸甲酯、油酸和二油酰磷脂酰胆碱的薄膜与2%的四氧化锇水溶液在0℃下反应1小时。作为对照,将9,10 - 二羟基硬脂酸甲酯和9,10 - 二羟基硬脂酸在室温下于甲醇中的0.25N氢氧化钠溶液中与四氧化锇反应1小时。分离、纯化反应产物,并通过薄层色谱、气液色谱以及红外和可见光谱进行分析。在所有情况下,产物均被鉴定为锇酸二酯,其中两个脂肪酸分子通过1个锇酸分子相连。