四氧化锇与不饱和脂质反应产物的色谱及分光光度研究
A chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids.
作者信息
Korn E D
出版信息
J Cell Biol. 1967 Aug;34(2):627-38. doi: 10.1083/jcb.34.2.627.
Abstract
Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0 degrees . As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid.
摘要
将油酸甲酯、油酸和二油酰磷脂酰胆碱的薄膜与2%的四氧化锇水溶液在0℃下反应1小时。作为对照，将9,10 - 二羟基硬脂酸甲酯和9,10 - 二羟基硬脂酸在室温下于甲醇中的0.25N氢氧化钠溶液中与四氧化锇反应1小时。分离、纯化反应产物，并通过薄层色谱、气液色谱以及红外和可见光谱进行分析。在所有情况下，产物均被鉴定为锇酸二酯，其中两个脂肪酸分子通过1个锇酸分子相连。
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本文引用的文献
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STUDIES ON THE REACTION OF OSMIUM TETROXIDE WITH LIPIDS AND RELATED COMPOUNDS.四氧化锇与脂质及相关化合物反应的研究
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Some electron microscopical observations on liquid-crystalline phases in lipid-water systems.关于脂质 - 水体系中液晶相的一些电子显微镜观察
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Fixation in cytochemistry and electron-microscopy.细胞化学与电子显微镜检查中的固定
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The use of osmium in the fixation and staining of tissues.锇在组织固定和染色中的应用。
Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):185-99. doi: 10.1098/rspb.1957.0043.
6
3. Modification of oleic acid during fixation of Amoebae by osmium tetroxide.3. 四氧化锇固定变形虫过程中油酸的修饰。
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7
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J Lipid Res. 1964 Jul;5(3):448-52.
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