• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Phosphorus nuclear-magnetic-resonance studies of the transition-state analogue complex of creatine kinase.肌酸激酶过渡态类似物复合物的磷核磁共振研究。
Biochem J. 1977 Dec 1;167(3):827-9. doi: 10.1042/bj1670827.
2
Combination of 31P-NMR magnetization transfer and radioisotope exchange methods for assessment of an enzyme reaction mechanism: rate-determining steps of the creatine kinase reaction.用于评估酶反应机制的31P-核磁共振磁化转移与放射性同位素交换方法的结合：肌酸激酶反应的速率决定步骤
Biochim Biophys Acta. 1990 Dec 6;1020(3):290-304. doi: 10.1016/0005-2728(90)90160-6.
3
31P NMR of enzyme-bound substrates of rabbit muscle creatine kinase. Equilibrium constants, interconversion rates, and NMR parameters of enzyme-bound complexes.兔肌酸激酶酶结合底物的31P核磁共振。酶结合复合物的平衡常数、相互转化率和核磁共振参数。
J Biol Chem. 1981 Feb 25;256(4):1716-21.
4
Determination of the affinity of each component of a composite quaternary transition-state analogue complex of creatine kinase.肌酸激酶复合季铵过渡态类似物复合物各组分亲和力的测定。
Biochemistry. 2002 Jun 4;41(22):6995-7000. doi: 10.1021/bi020105+.
5
Structure of metal-nucleotide complexes bound to creatine kinase: 31P NMR measurements using Mn(II) and Co(II).
Biochemistry. 1985 Jul 2;24(14):3487-94. doi: 10.1021/bi00335a015.
6
Binding of adenosine 5'-diphosphate to creatine kinase. An investigation using intermolecular nuclear Overhauser effect measurements.腺苷5'-二磷酸与肌酸激酶的结合。一项使用分子间核Overhauser效应测量的研究。
Biochemistry. 1976 Oct 19;15(21):4724-30. doi: 10.1021/bi00666a029.
7
Two-dimensional transferred nuclear Overhauser effect spectroscopy (TRNOESY) studies of nucleotide conformations in creatine kinase complexes: effects due to weak nonspecific binding.肌酸激酶复合物中核苷酸构象的二维转移核Overhauser效应光谱（TRNOESY）研究：弱非特异性结合的影响
Biochemistry. 1993 Nov 30;32(47):12941-8. doi: 10.1021/bi00210a049.
8
Magnetic resonance studies of specificity in binding and catalysis of phosphotransferases.磷酸转移酶结合与催化特异性的磁共振研究。
Ciba Found Symp. 1975(31):87-104. doi: 10.1002/9780470720134.ch6.
9
Structural changes of mitochondrial creatine kinase upon binding of ADP, ATP, or Pi, observed by reaction-induced infrared difference spectra.通过反应诱导红外差光谱观察到的线粒体肌酸激酶在结合ADP、ATP或磷酸根时的结构变化。
Biochemistry. 2001 Mar 6;40(9):2988-94. doi: 10.1021/bi002553s.
10
Asparagine 285 plays a key role in transition state stabilization in rabbit muscle creatine kinase.天冬酰胺285在兔肌肉肌酸激酶的过渡态稳定中起关键作用。
Protein Sci. 2003 Mar;12(3):532-7. doi: 10.1110/ps.0230403.

本文引用的文献

1
Thermodynamic studies of the formation and ionization of the magnesium(II) complexes of ADP and ATP over the pH range 5 to 9.在pH值5至9范围内对ADP和ATP的镁（II）配合物的形成和电离进行的热力学研究。
J Am Chem Soc. 1966 Jun 20;88(12):2631-40. doi: 10.1021/ja00964a002.
2
Inhibition of adenosine 5'-triphosphate-creatine phosphotransferase by substrate-anion complexes. Evidence for the transition-state organization of the catalytic site.底物 - 阴离子复合物对腺苷5'-三磷酸 - 肌酸磷酸转移酶的抑制作用。催化位点过渡态组织的证据。
Biochem J. 1971 May;122(5):727-40. doi: 10.1042/bj1220727.
3
The interaction of 8-anilino-1-naphthalenesulfonate with creatine kinase. Evidence for cooperativitiy of nucleotide binding.8-苯胺基-1-萘磺酸盐与肌酸激酶的相互作用。核苷酸结合协同性的证据。
J Biol Chem. 1974 Mar 10;249(5):1445-52.
4
Binding of adenosine 5'-diphosphate to creatine kinase. An investigation using intermolecular nuclear Overhauser effect measurements.腺苷5'-二磷酸与肌酸激酶的结合。一项使用分子间核Overhauser效应测量的研究。
Biochemistry. 1976 Oct 19;15(21):4724-30. doi: 10.1021/bi00666a029.
5
Creatine kinase. Modification of the working enzyme.肌酸激酶。活性酶的修饰。
Biochem J. 1976 Jul 1;157(1):23-31. doi: 10.1042/bj1570023.
6
Non-identical behaviour of the subunits of rabbit muscule creatine kinase.兔肌肉肌酸激酶亚基的不同行为
Biochim Biophys Acta. 1976 Sep 14;445(2):364-76. doi: 10.1016/0005-2744(76)90090-5.
7
Heterogeneity of rabbit muscle creatine kinase and limited proteolysis by proteinase K.兔肌肉肌酸激酶的异质性及蛋白酶K的有限蛋白水解作用
Biochem J. 1977 Dec 1;167(3):731-7. doi: 10.1042/bj1670731.
8
31P NMR studies of the arginine kinase reaction. Equilibrium constants and exchange rates at stoichiometric enzyme concentration.
J Biol Chem. 1976 Nov 25;251(22):6981-6.
9
31P nuclear magnetic resonance of bound substrates of arginine kinase reaction: chemical shifts in binary, ternary, quaternary, and transition state analog complexes.精氨酸激酶反应结合底物的31P核磁共振：二元、三元、四元及过渡态类似物复合物中的化学位移
J Biol Chem. 1977 May 25;252(10):3344-50.
10
Transition state analog inhibitors and enzyme catalysis.过渡态类似物抑制剂与酶催化
Annu Rev Biophys Bioeng. 1976;5:271-306. doi: 10.1146/annurev.bb.05.060176.001415.

肌酸激酶过渡态类似物复合物的磷核磁共振研究。

Phosphorus nuclear-magnetic-resonance studies of the transition-state analogue complex of creatine kinase.

作者信息

Milner-White E J, Rycroft D S

出版信息

Biochem J. 1977 Dec 1;167(3):827-9. doi: 10.1042/bj1670827.

DOI:10.1042/bj1670827
PMID:603637
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1183731/
Abstract

31P nuclear-magnetic-resonance spectra of MgADP bound to creatine kinase in the presence of creatine NO3- ions show that there are two non-identical forms of the bound nucleotide. The sites have different affinities for the nucleotide. MgADP at the high-affinity site is in slow exchange (k less than 125s-1 at 27 degrees C) with free MgADP.

摘要

在存在硝酸肌酸离子的情况下，与肌酸激酶结合的MgADP的31P核磁共振谱表明，结合的核苷酸存在两种不同形式。这些位点对核苷酸具有不同的亲和力。高亲和力位点的MgADP与游离MgADP处于缓慢交换状态（27℃时k小于125s-1）。