肌酸激酶过渡态类似物复合物的磷核磁共振研究。
Phosphorus nuclear-magnetic-resonance studies of the transition-state analogue complex of creatine kinase.
作者信息
Milner-White E J, Rycroft D S
出版信息
Biochem J. 1977 Dec 1;167(3):827-9. doi: 10.1042/bj1670827.
Abstract
31P nuclear-magnetic-resonance spectra of MgADP bound to creatine kinase in the presence of creatine NO3- ions show that there are two non-identical forms of the bound nucleotide. The sites have different affinities for the nucleotide. MgADP at the high-affinity site is in slow exchange (k less than 125s-1 at 27 degrees C) with free MgADP.
摘要
在存在硝酸肌酸离子的情况下，与肌酸激酶结合的MgADP的31P核磁共振谱表明，结合的核苷酸存在两种不同形式。这些位点对核苷酸具有不同的亲和力。高亲和力位点的MgADP与游离MgADP处于缓慢交换状态（27℃时k小于125s-1）。
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