尾草履虫中的微核RNA合成。
Micronuclear RNA synthesis in Paramecium caudatum.
作者信息
Narasimharao M V, Prescott D M
出版信息
J Cell Biol. 1967 May;33(2):281-5. doi: 10.1083/jcb.33.2.281.
Abstract
In a generation time of 8 hr in Paramecium caudatum, the bulk of DNA synthesis detected by thymidine-(3)H incorporation takes place in the latter part of the cell cycle. The micronuclear cycle includes a G(1) of 3 hr followed by an S period of 3-3(1/2) hr. G(2) and division occupies the remaining period of the cycle. Macronuclear RNA synthesis detected by 5'-uridine-(3)H incorporation is continuous throughout the cell cycle. Micronuclear RNA synthesis is restricted to the S period. Ribonuclease removes 80-90% of the incorporated label. Pulse-chase experiments showed that part of the RNA is conserved and released to the cytoplasm during the succeeding G(1) period.
摘要
在尾草履虫8小时的一代时间内，通过掺入氚标记胸腺嘧啶核苷检测到的大部分DNA合成发生在细胞周期的后期。小核周期包括3小时的G1期，随后是3 - 3.5小时的S期。G2期和分裂占据了周期的剩余时间。通过掺入5'-氚标记尿苷检测到的大核RNA合成在整个细胞周期中持续进行。小核RNA合成仅限于S期。核糖核酸酶去除80 - 90%的掺入标记。脉冲追踪实验表明，部分RNA在随后的G1期被保留并释放到细胞质中。
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